Nebularine
(Synonyms: 9-(Β-D-呋喃核糖)嘌呤) 目录号 : GC47760
A purine ribonucleoside with diverse biological activities
Cas No.:550-33-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Nebularine is a purine ribonucleoside and an adenosine analog that has been found in L. nebularis and has diverse biological activities.1,2,3,4,5 It reduces herpes simplex virus 1 (HSV-1) viral plaque formation in Vero cells (IC50 = 0.6 µM) and is active against M. phlei, M. avium, M. tuberculosis, and B. abortus.1,2 Nebularine (10 and 100 µM) is cytotoxic to COS-7 cells and induces pyknosis in mouse sarcoma 180 cells and mouse embryonic skin explants in a concentration-dependent manner.3,4 It inhibits root growth of wheat seedlings when used at concentrations ranging from 0.1 to 2 mM.2 Nebularine also inhibits adenosine deaminase (Ki = 16 µM).5
1.JuliÁn-Ortiz, J.V., GÁlvez, J., MuÑoz-Collado, C., et al.Virtual combinatorial syntheses and computational screening of new potential anti-herpes compoundsJ. Med. Chem.42(17)3308-3314(1999) 2.Brown, E.G., and Konuk, M.Plant cytotoxicity of nebularine (purine riboside)Phytochemistry37(6)1589-1592(1994) 3.Tkacz, K., Cioroch, M., Skladanowski, A.C., et al.The cytotoxic effect of purine riboside on COS-7 cellsPurine and Pyrimidine Metabolism in Man X. Advances in Experimental Medicine and Biology486355-359(2000) 4.Biesele, J.J., Slautterback, C., and Margolis, M.Unsubstituted purine and its riboside as toxic antimetabolites in mouse tissue culturesCancer8(1)87-96(1955) 5.Shewach, D.S., Krawczyk, S.H., Acevedo, O.L., et al.Inhibition of adenosine deaminase by azapurine ribonucleosidesBiochem. Pharmacol.44(9)1697-1700(1992)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.9651 mL | 19.8255 mL | 39.6511 mL |
| 5 mM | 0.793 mL | 3.9651 mL | 7.9302 mL |
| 10 mM | 0.3965 mL | 1.9826 mL | 3.9651 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。