Neopuerarin A
(Synonyms: 新葛根甲素) 目录号 : GC61121
NeopuerarinA是从葛根干根的水提取物中分离得到的异黄酮。NeopuerarinA显示出有效的肝保护作用。
Cas No.:1150314-34-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Neopuerarin A is an isoflavones isolated from the water extraction of the dried roots of Pueraria lobata (Willd.). Neopuerarin A shows significant hepatoprotective effect[1].
[1]. Yingjie Sun, et al. New hepatoprotective isoflavone glucosides from Pueraria lobata (Willd.) Ohwi. Nat Prod Res. 2019 Dec;33(24):3485-3492.
| Cas No. | 1150314-34-3 | SDF | |
| 别名 | 新葛根甲素 | ||
| Canonical SMILES | O=C1C(C2=CC=C(O)C=C2)=COC3=C([C@@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O)O4)O)O)C(O)=CC=C13 | ||
| 分子式 | C21H20O9 | 分子量 | 416.38 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4017 mL | 12.0083 mL | 24.0165 mL |
| 5 mM | 0.4803 mL | 2.4017 mL | 4.8033 mL |
| 10 mM | 0.2402 mL | 1.2008 mL | 2.4017 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
New hepatoprotective isoflavone glucosides from Pueraria lobata (Willd.) Ohwi
Nat Prod Res 2019 Dec;33(24):3485-3492.PMID:29968479DOI:10.1080/14786419.2018.1484461.
Two new isoflavone glucosides, 3'-methoxyneopuerarin A (1) and 3'-methoxyneopuerarin B (2), together with nine known isoflavones including puerarin (3), Neopuerarin A (4), neopuerarin B (5), daidzin (6), daidzein (7), 3'-methoxypuerarin (PG-3) (8), puerarin xyloside (9), mirificin (10), 3'-hydroxypuerarin (11) were isolated from the water extraction of the dried roots of Pueraria lobata (Willd.) Ohwi. Their structures were elucidated by the means of spectroscopic and chromatographic analysis methods. All compounds were evaluated for their hepatoprotective activity on HepG2 cells. All of them showed statistically significant hepatoprotective effect.
Isolation of two new C-glucofuranosyl isoflavones from Pueraria lobata (Wild.) Ohwi with HPLC-MS guiding analysis
J Asian Nat Prod Res 2010 Apr;12(4):293-9.PMID:20419540DOI:10.1080/10286021003745445.
A profiling analysis of the total isoflavone extract of the root of Pueraria lobata (Wild.) Ohwi was performed using HPLC coupled with ESI-ion trap mass spectrometry. A total of seven isoflavones were identified according to their retention times, UV, MS data, and comparing with the literature data. Among them, two proposed new compounds were isolated and their structures were determined to be 8-C-alpha-glucofuranosyl-7,4'-dihydroxyisoflavone and 8-C-beta-glucofuranosyl-7,4'-dihydroxyisoflavone, named as Neopuerarin A (7) and neopuerarin B (6), on the basis of chemical and spectral analyses.
Profiling and quantification of isoflavone-C-glycosides impurities in puerarin injection by liquid chromatography coupled to ESI-ion trap mass spectrometry
J Pharm Biomed Anal 2009 Apr 5;49(3):843-7.PMID:19186018DOI:10.1016/j.jpba.2008.12.033.
An HPLC/DAD/MS(n) method was established for the qualitative and quantitative analysis of the impurities in puerarin injection (PI), a widely used drug in China. The analytical HPLC was performed on an Agela RP-C18 column using 0.1% aqueous formic acid (v:v) and methanol as mobile phase. A total of nine impurities were detected and eight of them were identified as isoflavone-C-glycosides basing on their UV spectra and MS(n) spectra and comparing with the literature data. An HPLC method for the assay of two common impurities in the commercial PI samples, i.e., Neopuerarin A and neopuerarin B, was then established. The validation of the method, including sensitivity, linearity, precision, accuracy, was carried out. The calibration curves showed good linearity of R(2)>0.9999 and LOQ (S/N=10) were less than 3.73 ng. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 0.94%. The average recovery rates were 97.0% and 99.5%, respectively, with R.S.D. less than 1.38%. The contents of Neopuerarin A and neopuerarin B in various commercial brands of PI samples varied over the range of 0.30-1.16% and 0.42-1.66%, respectively. This is the first report on the impurities in PI.