Neuropeptide EI (rat) (trifluoroacetate salt)
(Synonyms: NEI; Neuropeptide-Glutamic Acid-Isoleucine) 目录号 : GC92100Neuropeptide EI (rat) (trifluoroacetate salt)是大鼠前黑色素浓缩激素MCH的内源性肽片段，参与激素释放、梳理行为和运动活动。
Sample solution is provided at 25 µL, 10mM.
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Neuropeptide EI (NEI) is an endogenous peptide fragment of rat prepro-melanin concentrating hormone (MCH) that is involved in hormone release, grooming behavior, and motor activity.1 NEI corresponds to amino acids 140-152 of rat prepro-MCH and 131-143 of human, mouse, and rat prepro-MCH. It is expressed in both the central and peripheral nervous systems, localizes to the cytoplasm, and colocalizes with MCH in the CNS.1,2 NEI (1 ?M) reduces thyrotropin-releasing hormone (TRH) release in mediobasal hypothalamus explants.3 Intracerebroventricular administration of NEI increases serum levels of luteinizing hormone (LH) in male rats and in chronically ovariectomized female rats that received estradiol benzoate and progesterone in addition to NEI.4 NEI (1 ?g/animal, i.c.v.) induces excessive grooming behavior and increases locomotor activity in rats, effects that can be blocked by MCH or α-melanocyte-stimulating hormone (α-MSH).5 It also increases the amount of time spent in REM sleep in rats when injected intracerebroventricularly or into the ventrolateral periaqueductal grey (vlPAG).
References:
[1]. Bittencourt, J., and Celis, M.E.Anatomy, function and regulation of neuropeptide EI (NEI)Peptides29(8)1441-1450(2008).
[2]. Bittencourt, J.C.Anatomical organization of the melanin-concentrating hormone peptide family in the mammalian brainGen. Comp. Endocrinol.172(2)185-197(2011).
[3]. Kennedy, A.R., Todd, J.F., Stanley, S.A., et al.Melanin-concentrating hormone (MCH) suppresses thyroid stimulating hormone (TSH) release, in vivo and in vitro, via the hypothalamus and the pituitaryEndocrinology42(7)3265-3268(2001).
[4]. Attademo, A.M., Sánchez-Borzone, M., Lasaga, M., et al.Intracerebroventricular injection of neuropeptide EI increases serum LH in male and female ratsComparative Study25(11)1995-1999(2004).
[5]. Sanchez, M., Baker, B.I., and Celis, M.Melanin-concentrating hormone (MCH) antagonizes the effects of α-MSH and neuropeptide E-I on grooming and locomotor activities in the ratPeptides18(3)393-396(1997).
| Cas No. | SDF | ||
| 别名 | NEI; Neuropeptide-Glutamic Acid-Isoleucine | ||
| 化学名 | L-α-glutamyl-L-isoleucylglycyl-L-α-aspartyl-L-α-glutamyl-L-α-glutamyl-L-asparaginyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-L-prolyl-L-isoleucinamide, trifluoroacetate salt | ||
| Canonical SMILES | OC(C(F)(F)F)=O.[H]N[C@@H](CCC(O)=O)C(N[C@]([C@H](CC)C)([H])C(NCC(N[C@H](C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CC(N)=O)C(N[C@@H](CO)C(N[C@H](C(N[C@@H](CCCCN)C(N[C@H](C(N1CCC[C@H]1C(N[C@]([C@H](CC)C)([H])C(N)=O)=O)=O)CC2=CC=CC=C2)=O)=O)C)=O)=O)=O)=O)=O)CC(O)=O)=O)=O)=O | ||
| 分子式 | C63H98N16O23 ? XCF3COOH | 分子量 | 1447.6 |
| 溶解度 | DMSO: Sparingly soluble: 1-10 mg/ml,PBS (pH 7.2): Soluble: ≥10 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.6908 mL | 3.454 mL | 6.908 mL |
| 5 mM | 0.1382 mL | 0.6908 mL | 1.3816 mL |
| 10 mM | 0.0691 mL | 0.3454 mL | 0.6908 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet