Neuropeptide Y (scrambled)
目录号 : GC10695
Control peptide for neuropeptide Y (NPY)
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Scrambled Neuropeptide Y (scNPY) was similarly synthesized and contains the same amino acids as NPY, but scNPY is in a random sequence, it was used as a control in the research in NPY [1]. The sequence of scNPY is SKPQRDANREPTRYAIYDYSNPDIELHYLRPAYALG-NH2 [2].
Neuropeptide Y (NPY) is a 36-amino acid neuropeptide that exerts its activity via G-protein-coupled receptors. NPY is widely distributed in the peripheral and central nervous system. It modulates a variety of physiological processes, e.g. the central regulation of vasoconstriction, memory retention and food intake, and the regulation of circadian rhythm [2].
Scrambled NPY peptide treatment had no effect on VEGF expression in 4T1 cells. Previous treatment with scrambled NPY at concentrations ranging from 10−10 to 10−7 M made conditioned media from 4T1 cells show no difference in the number of complete endothelial networks or branch points versus control in endothelial tube formation assays [3].
In trimethyltin-treated (TMT-treated) rats, treatment with scrambled NPY for 24 h made the expression of Bdnf, NpyR, neurogenic and antiapoptotic genes comparable to that of saline-injected animals (without significant differences) [4]. In TMT-treated rats, both saline and scNPY showed no significant modulation (p> 0.05) in the expression levels of Npy1R, Npy2R and Npy5R [5].
References:
[1]. Bouchaïb El Bahh, Silvia Balosso, Trevor Hamilton, et al. The anti-epileptic actions of neuropeptide Y in the hippocampus are mediated by Y2 and not Y5 receptors. European Journal of Neuroscience, 2005, 22: 1417-1430.
[2]. Daniela Proske, Martin Hőfliger, Richard M. Sőll, et al. A Y2 Receptor Mimetic Aptamer Directed against Neuropeptide Y. The Journal of Biological Chemistry, 2002, 277(13):11416-11422.
[3]. Philip J. Medeirosa and Dwayne N. Jackson. Neuropeptide Y Y5-receptor activation on breast cancer cells acts as a paracrine system that stimulates VEGF expression and secretion to promote angiogenesis. Peptides, 2013, 48:106-113.
[4]. Valentina Corvino, Elisa Marchese, Stefano Giannetti, et al. The neuroprotective and neurogenic effects of neuropeptide Y administration in an animal model of hippocampal neurodegeneration and temporal lobe epilepsy induced by trimethyltin. J. Neurochem., 2012, 122: 415-426.
[5]. Valentina Corvino, Elisa Marchese, Maria Vittoria Podda, et al. The Neurogenic Effects of Exogenous Neuropeptide Y: Early Molecular Events and Long-lasting Effects in the Hippocampus of Trimethyltin-Treated Rats. PLOS ONE, 2014, 9(2): e88294.
| Cas No. | SDF | ||
| 分子式 | C190H287N55O57 | 分子量 | 4253.7 |
| 溶解度 | Soluble to 1 mg/ml in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.2351 mL | 1.1754 mL | 2.3509 mL |
| 5 mM | 0.047 mL | 0.2351 mL | 0.4702 mL |
| 10 mM | 0.0235 mL | 0.1175 mL | 0.2351 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。