NG-amino-L-Arginine (hydrochloride)
目录号 : GC15316Inhibitor of NOS enzymes
Cas No.:1031799-40-2
Sample solution is provided at 25 µL, 10mM.
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- Purity: >98.00%
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- SDS (Safety Data Sheet)
- Datasheet
NG-amino-L-arginine, a novel structural analog of L-arginine, is an inhibitor of nitric oxide synthase (NOS) [1,2]. Nitric oxide synthases (NOSs) have been involved in catalyzing the production of nitric oxide (NO) from L-arginine. As an important cellular signaling molecule, NO has been implicated in modulating vascular tone, airway tone, insulinsecretion, and peristalsis. It has also been shown that NO is involved in angiogenesis and neural development and can function as a retrograde neurotransmitter [3].
In vitro: NG-amino-L-arginine potently and stereoselectively induced endothelium-dependent contraction. NG-amino-L-arginine caused concentration-dependent, competitive, and stereoselective antagonism of acetylcholine-elicited relaxation and cyclic GMP accumulation. NG-Amino-L-arginine was 100- to 300- fold more potent than NG-methyl-L-arginine [1]. NG-amino-L-arginine (100 μM) almost abolished endothelium-dependent relaxation induced by acetylcholine, but was rapidly restored by addition of 300 μM L-arginine. The maximal response to acetylcholine was inhibited by NG-amino-L-arginine in excess of 1 μM and was abolished by concentrations in the range of l0-30 μM [1]. NG-Amino-L-arginine inactivated the citrulline-forming activity of the nNOS, iNOS, and eNOS isoforms with the maximal inactivation rates of 0.35, 0.26, and 0.53 min-1 and Ki values of 0.3, 3, and 2.5 μM, respectively [2].
In vivo: In awake animal models of sepsis, treatment with NG-amino-L-arginine showed higher systemic and pulmonary vascular resistance indices and decreased heart rates, cardiac indices, oxygen delivery indices, and oxygen consumption indices when compared with controls [4]. NG-amino-L-arginine increased mortality rates after endotoxin challenge [4].
References:
[1] Fukuto J M, Wood K S, Byrns R E, et al. NG-amino-L-arginine: a new potent antagonist of L-arginine-mediated endothelium-dependent relaxation[J]. Biochemical and biophysical research communications, 1990, 168(2): 458-465.
[2] Wolff D J, Lubeskie A. Inactivation of Nitric Oxide Synthase Isoforms by Diaminoguanidine andN G-Amino-L-arginine[J]. Archives of biochemistry and biophysics, 1996, 325(2): 227-234.
[3] Frstermann U, Closs E I, Pollock J S, et al. Nitric oxide synthase isozymes. Characterization, purification, molecular cloning, and functions[J]. Hypertension, 1994, 23(6 Pt 2): 1121-1131.
[4] Cobb J P, Natanson C, Hoffman W D, et al. N omega-amino-L-arginine, an inhibitor of nitric oxide synthase, raises vascular resistance but increases mortality rates in awake canines challenged with endotoxin[J]. Journal of Experimental Medicine, 1992, 176(4): 1175-1182.
Cas No. | 1031799-40-2 | SDF | |
化学名 | N5-(hydrazinyliminomethyl)-L-ornithine, monohydrochloride | ||
Canonical SMILES | NNC(NCCC[C@H](N)C(O)=O)=N.Cl | ||
分子式 | C6H15N5O2 • HCl | 分子量 | 225.7 |
溶解度 | ≤10mg/ml in PBS(pH7.2) | 储存条件 | Store at -20°C |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 4.4307 mL | 22.1533 mL | 44.3066 mL |
5 mM | 0.8861 mL | 4.4307 mL | 8.8613 mL |
10 mM | 0.4431 mL | 2.2153 mL | 4.4307 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
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1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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