Nicotinamide riboside
(Synonyms: 烟酰胺核糖) 目录号 : GC44401
烟酰胺核苷是维生素 B3 和 NAD+ 前体的一种形式，通过烟酰胺核苷激酶 (NRK) 和 NMNAT，或通过核苷磷酸化酶和 NAM 补救作用，转化为生物可利用的 NAD+.在体外实验中，烟酰胺核苷通过激活 SirT1/PGC-1α/线粒体生物合成途径减轻酒精引起的肝损伤。
Cas No.:1341-23-7
Sample solution is provided at 25 µL, 10mM.
Nicotinamide riboside, a form of vitamin B3 and NAD+ precursor, is converted to bioavailable NAD+, via nicotinamide riboside kinase (NRK) and NMNAT, or by the action of nucleoside phosphorylase and NAM salvage[1-2].
In in vitro experiments ,nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway [3]. Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans[4].
Nicotinamide Riboside was able to enhance the skeletal muscle NAD+ metabolome, inducing gene expression signatures implicated in downregulation of energy metabolism pathways, but did not affect muscle mitochondrial bioenergetics or metabolism[5]. Nicotinamide Riboside enhances deacetylase activity in vivo, deacetylates PGC-1α in muscle, liver, and BAT, and it induces deacetylase activity in tissues where NAD+ accumulates[6].
References:
[1] Bieganowski P., Brenner C. Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans. Cell.2004;117:495–502.
[2] Nikiforov A., Dolle C., Niere M. Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation. J. Biol. Chem. 2011;286:21767–21778.
[3] Wang S, Wan T, et al. Nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway. Redox Biol. 2018 Jul;17:89-98.
[4] Nascimento EBM, Moonen MPB, et al. Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans. J Clin Endocrinol Metab. 2021 Apr 23;106(5):1437-1447.
[5] Elhassan YS, Kluckova K, et al. Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD+ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures. Cell Rep. 2019 Aug 13;28(7):1717-1728.e6.
[6] Cantó C, Houtkooper RH,et al. The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity. Cell Metab. 2012 Jun 6;15(6):838-47.
烟酰胺核苷是维生素 B3 和 NAD+ 前体的一种形式,通过烟酰胺核苷激酶 (NRK) 和 NMNAT,或通过核苷磷酸化酶和 NAM 补救作用,转化为生物可利用的 NAD+[1-2].
在体外实验中,烟酰胺核苷通过激活 SirT1/PGC-1α/线粒体生物合成途径减轻酒精引起的肝损伤[3]。烟酰胺核苷增强人体体外 β-肾上腺素能棕色脂肪组织活性[4]。
烟酰胺核苷能够增强骨骼肌 NAD+ 代谢组,诱导与能量代谢途径下调有关的基因表达特征,但不影响肌肉线粒体生物能量学或代谢[5]。烟酰胺核苷在体内增强脱乙酰酶活性,使肌肉、肝脏和BAT中的PGC-1α脱乙酰化,并在NAD+积累的组织中诱导脱乙酰酶活性[6]。
| Cell experiment [1]: | |
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Cell lines |
Murine RAW 264.7 macrophages |
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Preparation Method |
The prepared mouse bone marrow-derived macrophages were cultured and stored at 37°C in 5% CO2. Murine RAW 264.7 macrophages were treated with EtOH (80 mM), MeCHO (200 μM), and nicotinamide riboside (1 mM), respectively, and ROS accumulation was determined. |
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Reaction Conditions |
1 mM ,72 h |
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Applications |
Ethanol and its metabolite, acetaldehyde, significantly increased cellular ROS levels, but Nicotinamide riboside completely abolished the increase to a basal level in RAW 264.7 macrophages. |
| Animal experiment [2]: | |
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Animal models |
Eight-week-old male C57BL/6 J mice |
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Preparation Method |
The reared mice were randomly divided into 3 groups: control group (CTRL), ethanol group (EtOH) and nicotinamide riboside supplementation group (EtOH+ Nicotinamide riboside ). Mice in the ethanol and nicotinamide riboside supplemented groups were fed a Lieber-DeCarli ethanol liquid diet, while control mice were paired as previously described. |
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Dosage form |
400 mg/kg, oral gavage once daily for 10 consecutive days |
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Applications |
Mice were fed in pairs and there was no difference in body weight between the three groups. Fat accumulation was observed in the ethanol group, while only a few tiny lipid droplets were observed in the nicotinamide riboside group. Nicotinamide riboside significantly decreased serum ALT and AST and liver triglyceride levels, and slightly decreased liver weight ratio. |
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References: [1]. Wang S, Wan T,et al. Nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway. Redox Biol. 2018 Jul;17:89-98. [2]. Kang H, Park YK, Lee JY. Nicotinamide riboside, an NAD+ precursor, attenuates inflammation and oxidative stress by activating sirtuin 1 in alcohol-stimulated macrophages. Lab Invest. 2021 Sep;101(9):1225-1237. |
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| Cas No. | 1341-23-7 | SDF | |
| 别名 | 烟酰胺核糖 | ||
| Canonical SMILES | NC(C1=C[N+]([C@H]2[C@H](O)[C@H](O)[C@@H](CO)O2)=CC=C1)=O | ||
| 分子式 | C11H15N2O5 | 分子量 | 255.3 |
| 溶解度 | Soluble in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.917 mL | 19.5848 mL | 39.1696 mL |
| 5 mM | 0.7834 mL | 3.917 mL | 7.8339 mL |
| 10 mM | 0.3917 mL | 1.9585 mL | 3.917 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
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Quality Control & SDS
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- Purity: >99.50%
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