Nicotine
(Synonyms: (+/-)-尼古丁,(±)-Nicotine) 目录号 : GC14043An agonist at neuronal nicotinic acetylcholine receptors
Cas No.:22083-74-5
Sample solution is provided at 25 µL, 10mM.
-
Related Biological Data
Nicotine activates the prok2R/Akt/FoxO3a axis and exhibits anti-apoptotic effects against MPP+in the HEK293T cell line.(e) and semi-quantitative analysis of fluorescence intensity
Nicotine (GLPBIO) was dissolved in DMSO to form the concentration of 20 mg/100 μL. Then the solution was diluted to 10 mL using saline to 2 mg/mL.
J Transl Med 22.1 (2024): 1-21. PMID: 38609979 IF: 7.4001 -
Related Biological Data
The role of Met in hyperlipidaemia-associated vascular calcification.(A) Experimental design: SD rats in four groups were subjected to the indicated treatment for 6 weeks in total. (B) Representative Von Kossa and H&E staining in aortic tissues of SD rats (scale bars = 500 μm).
To establish a vascular calcification model, rats were injected with vitamin D3 (300,000 IU/kg) and simultaneously intragastrically administered nicotine (25 mg/kg in 3 mL peanut oil) (GLPBIO)at 9a.m. on the first day.
Free Radical Biology and Medicine (2021). PMID: 33513420 IF: 7.373 -
Related Biological Data
OEA alleviates vascular calcification caused by hyperlipidemia in vivo and PA in vitro. (A) Schematic protocol of animal feed and treatment. (B, C)Representative Von Kossa (B) and H&E (C) staining on thoracic aorta slices of SD rats (Scale bars: 500 μm, n = 6–8).
Nicotine (GLPBIO)(25 mg/kg/day in 3 mL peanut oil) was injected, gavaged, respectively, and nicotine intervention was repeated after 8 h on the same day for inducing the model of vascular calcification.
Biochemical Pharmacology 208 (2023): 115379. PMID: 36525991 IF: 6.1004
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
(±)-Nicotine is the racemic mixture of the dominant alkaloid found in tobacco plants. It acts as an agonist at neuronal nicotinic acetylcholine receptors (nAChRs; Kis = 481 and 11.1 nM for α3β4 and α4β2 subtypes, respectively) and possesses addictive and teratogenic properties.1 (−)-(S)-Nicotine is significantly more active at binding nAChRs compared to the (+)-(R) antipode, thus nicotine is typically synthesized as (−)-(S)-nicotine with only 0.2-1% of the (+)-(R) isomer present.2
References
1. Zaveri, N., Jiang, F., Olsen, C., et al. Novel α3β4 nicotinic acetylcholine receptor-selective ligands. Discovery, structure-activity studies, and pharmacological evaluation. J. Med. Chem. 53(22), 8187-8191 (2010).
2. Clayton, P., Lu, A., and Bishop, L. The pyrolysis of (−)-(S)-nicotine: Racemization and decomposition. Chirality 22(4), 442-446 (2010).
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 6.1641 mL | 30.8204 mL | 61.6409 mL |
| 5 mM | 1.2328 mL | 6.1641 mL | 12.3282 mL |
| 10 mM | 0.6164 mL | 3.082 mL | 6.1641 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。