Nigericin
(Synonyms: 尼日利亚霉素) 目录号 : GC39719
A potassium ionophore
Cas No.:28380-24-7
Sample solution is provided at 25 µL, 10mM.
Nigericin is an antibiotic derived from S. hygroscopicus that acts as a potassium ionophore promoting K+/H+ exchange across mitochondrial membranes. Nigericin can be used as a research tool to disrupt intracellular H+ and K+ concentration, thus altering pH, membrane potential, and cell volume.1,2,3 At 10 μM, nigericin induces egress of T. gondii parasites by inducing efflux of K+.4
Nigericin是一种来源于吸湿链霉菌的抗生素，作为K+/H+离子载体，促进线粒体膜上K+/H+[1]的交换。Nigericin可以是一种NLRP3激活剂，以NALP3依赖的方式诱导IL-1β的释放[2]。Nigericin引发丹毒，这种作用与ROS的形成平行，部分原因是氧化应激的诱导。Nigericin触发细胞凋亡[3]。
[1]. Zotova L, et al. Novel components of an active mitochondrial K(+)/H(+) exchange. J Biol Chem. 2010 May 7;285(19):14399-414.
[2]. Mariathasan S, et al.Cryopyrin activates the inflammasome in response to toxins and ATP.Nature. 2006 Mar 9;440(7081):228-32.
[3]. Bissinger R, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016 May;118(5):381-9.
| Cas No. | 28380-24-7 | SDF | |
| 别名 | 尼日利亚霉素 | ||
| Canonical SMILES | C[C@H]1[C@](O[C@@H]2C[C@](CC[C@@H]3C)([H])O[C@@]3([H])[C@@H](C)C(O)=O)([C@@H]([C@H](OC)C2)C)O[C@](C)([C@]4([H])O[C@](C)([C@]5([H])O[C@]([C@@]([C@H](C[C@H]6C)C)([H])O[C@@]6(O)CO)([H])C[C@@H]5C)CC4)C1 | ||
| 分子式 | C40H68O11 | 分子量 | 724.96 |
| 溶解度 | 1.2mg/mL in DMF, 20mg/mL in Ethanol | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3794 mL | 6.8969 mL | 13.7939 mL |
| 5 mM | 0.2759 mL | 1.3794 mL | 2.7588 mL |
| 10 mM | 0.1379 mL | 0.6897 mL | 1.3794 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Lysosomal accumulation and cellular pH-responsive capability of NRhD-PEG-4 NPs. (b) Fluorescence images of A549 and MCF-7 cells incubated with NRhD-PEG-4 NPs at different pH values (4.5, 5.5, 6.5, and 7.5). (c) Fluorescence quantification in (a).
For the intracellular pH-reactive test, the cells were treated with 1.0 mg/mL nigericin (GLPBIO) in a high K+ buffer of different pH values for 30 min.
Acta Biomaterialia (2022). PMID: 36087865 IF: 10.6335 -
Related Biological Data
IPI-2I cells were transfected with siR-SLC9A3, NC, pEGFP-SLC9A3 or pEGFP. (G-H) NHE3 activity were analyzed by using pH sensitive dye BCECF-AM.
Finally, the re-addition of the Na+-solution and measurement of the pH at the end of each experiment, thefluorescence ratio was calibrated to the appropriate pH using nigericin (2 μM; Glpbio,Shanghai, China)/high K+-solutions at different pH values.
Vet Microbiol 289 (2024): 109916. PMID: 38159369 IF: 3.3 -
Related Biological Data
Vitamin K3 Suppresses Pyroptosis in THP-1 Cells. Vitamin K3 (0, 5, 10, 20, and 30 μM) was used to pretreat THP-1 cells for 1 h before LPS plus nigericin (Nig) induced pyroptosis. (A) Cell viability and (B) supernatant LDH levels were quantified.
Briefly, 1 × 106/mL cells were cultured with RPMI 1640 medium containing 2% FBS overnight and then treated with 1 μg/mL LPS for 3 h, followed by 8 μg/mL Nigericin (GlpBio) treatment for 1 h.
Biol Pharm Bull 46.1 (2023): 52-60. PMID: 36288961 IF: 2.2641