Nimesulide D5
(Synonyms: 尼美舒利 D5) 目录号 : GC39583
An internal standard for the quantification of nimesulide
Cas No.:1330180-22-7
Sample solution is provided at 25 µL, 10mM.
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Nimesulide-d5 is intended for use as an internal standard for the quantification of nimesulide by GC- or LC-MS. Nimesulide is a non-steroidal anti-inflammatory drug (NSAID) and COX-2 inhibitor (IC50s = 1.27 and 0.03 ?M for the human and ovine enzymes, respectively).1,2 It is selective for COX-2 over COX-1 (IC50s = 70 and 22 ?M for the human and ovine enzymes, respectively). Nimesulide also inhibits sodium-dependent neutral amino acid transporter (B0AT1) with an IC50 value of 23 ?M for the rat kidney transporter.3 It inhibits infection-induced increases in brain prostaglandin E2 levels, as well as reduces pyresis (ED50 = 0.3 mg/kg), in yeast-infected rats.4 Nimesulide (2.9 mg/kg) inhibits formalin-induced hindpaw thermal hyperalgesia in rats.5
1.Johnson, J.L., Wimsatt, J., Buckel, S.D., et al.Purification and characterization of prostaglandin H synthase-2 from sheep placental cotyledonsArch. Biochem. Biophys.324(1)26-34(1995) 2.Barnett, J., Chow, J., Ives, D., et al.Purification, characterization and selective inhibition of human prostaglandin G/H synthase 1 and 2 expressed in the baculovirus systemBiochim. Biophys. Acta1209(1)130-139(1994) 3.Pochini, L., Seidita, A., Sensi, C., et al.Nimesulide binding site in the B0AT1 (SLC6A19) amino acid transporter. Mechanism of inhibition revealed by proteoliposome transport assay and molecular modellingBiochem. Pharmacol.89(3)422-430(2014) 4.Taniguchi, Y., Yokoyama, K., Inui, K., et al.Inhibition of brain cyclooxygenase-2 activity and the antipyretic action of nimesulideEur. J. Pharmacol.330221-229(1997) 5.Bianchi, M., and Broggini, M.Anti-hyperalgesic effects of nimesulide: Studies in rats and humansInt. J. Clin. Pract. Suppl.57(128)11-19(2002)
| Cas No. | 1330180-22-7 | SDF | |
| 别名 | 尼美舒利 D5 | ||
| Canonical SMILES | CS(=O)(NC1=CC=C([N+]([O-])=O)C=C1OC2=C([2H])C([2H])=C([2H])C([2H])=C2[2H])=O | ||
| 分子式 | C13H7D5N2O5S | 分子量 | 313.34 |
| 溶解度 | Methanol: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1914 mL | 15.9571 mL | 31.9142 mL |
| 5 mM | 0.6383 mL | 3.1914 mL | 6.3828 mL |
| 10 mM | 0.3191 mL | 1.5957 mL | 3.1914 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet