Ninhydrin
(Synonyms: 茚三酮) 目录号 : GC61135
Ninhydrin可作为显色分析探针(chromogenicanalyticalprobe)用于氨基酸和蛋白质的定量分析。
Cas No.:485-47-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.
Ninhydrin is used in amino acid analysis of proteins. Except proline, Most amino acids can be hydrolyzed and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by chromatography.Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine.
[1]. Omar MA, et al. Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma.Luminescence. 2018 Sep;33(6):1107-1112. [2]. Anantharaman S, et al. Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins.rSpectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:897-903.
| Cas No. | 485-47-2 | SDF | |
| 别名 | 茚三酮 | ||
| Canonical SMILES | O=C1C(O)(O)C(C2=C1C=CC=C2)=O | ||
| 分子式 | C9H6O4 | 分子量 | 178.14 |
| 溶解度 | DMSO : 50 mg/mL (280.68 mM; Need ultrasonic); H2O : 25 mg/mL (140.34 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.6136 mL | 28.0678 mL | 56.1356 mL |
| 5 mM | 1.1227 mL | 5.6136 mL | 11.2271 mL |
| 10 mM | 0.5614 mL | 2.8068 mL | 5.6136 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Ninhydrin-functionalized chitosan for selective removal of Pb(II) ions: Characterization and adsorption performance
Int J Biol Macromol 2021 Apr 30;177:29-39.PMID:33607139DOI:10.1016/j.ijbiomac.2021.02.110.
A chitosan-based adsorbents (CS-Ninhydrin) was prepared by grafting Ninhydrin for Pb(II) ions adsorption. SEM-EDS, XRD and FTIR analysis were used to characterize the synthesized CS-Ninhydrin. The static adsorption experiments showed that CS-Ninhydrin had a good removal rate for Pb(II) ions in a wide range of pH 3 to 7, quickly reached equilibrium (120 min) and had a higher adsorption capacity (196 mg/g). Pseudo second-order and Langmuir models showed that the adsorption process of Pb(II) by CS-Ninhydrin was a single-layer chemical adsorption. Temperature experiments showed that the reaction was a spontaneous exothermic process. In the wastewater experiment, CS-Ninhydrin showed an excellent selectivity to Pb(II) ions. The reusability of CS-Ninhydrin was perfect after five adsorption-desorption cycles. The main adsorption mechanism was the chelating and electrostatic action between N and O groups in CS-Ninhydrin and Pb(II) ions. Therefore, the new adsorbent CS-Ninhydrin was expected to promote the wide application of chitosan in Pb(II) adsorption.
The development of novel Ninhydrin analogues
Chem Soc Rev 2005 May;34(5):408-17.PMID:15852153DOI:10.1039/b315496n.
Following its discovery by Siegfried Ruhemann in 1910, Ninhydrin rapidly became a practical analytical tool. In 1954 it was found to be an important reagent to develop fingerprints on porous surfaces. Since its use in forensic chemistry, many efforts have focused on improving the reagent. Many of the shortcomings of Ninhydrin have been met by the synthesis of a variety of Ninhydrin analogues. This tutorial review provides a short introduction to Ninhydrin and highlights the different synthetic approaches used in the development of analogues for the detection of latent fingerprints.
A Ninhydrin-Type Urea Sorbent for the Development of a Wearable Artificial Kidney
Macromol Biosci 2020 Mar;20(3):e1900396.PMID:32065727DOI:10.1002/mabi.201900396.
The aim of this study is to develop polymeric chemisorbents with a high density of Ninhydrin groups, able to covalently bind urea under physiological conditions and thus potentially suitable for use in a wearable artificial kidney. Macroporous beads are prepared by suspension polymerization of 5-vinyl-1-indanone (vinylindanone) using a 90:10 (v/v) mixture of toluene and nitrobenzene as a porogen. The indanone groups are subsequently oxidized in a one-step procedure into Ninhydrin groups. Their urea absorption kinetics are evaluated under both static and dynamic conditions at 37 °C in simulated dialysate (urea in phosphate buffered saline). Under static conditions and at a 1:1 molar ratio of Ninhydrin: urea the sorbent beads remove ≈0.6-0.7 mmol g-1 and under dynamic conditions and at a 2:1 molar excess of Ninhydrin ≈0.6 mmol urea g-1 sorbent in 8 h at 37 °C, which is a step toward a wearable artificial kidney.
Applications of the Ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences
J Agric Food Chem 2004 Feb 11;52(3):385-406.PMID:14759124DOI:10.1021/jf030490p.
The reaction of Ninhydrin with primary amino groups to form the purple dye now called Ruhemann's purple (RP) was discovered by Siegfried Ruhemann in 1910. In addition, imines such as pipecolic acid and proline, the guanidino group of arginine, the amide groups of asparagine, the indole ring of tryptophan, the sulfhydryl group of cysteine, amino groups of cytosine and guanine, and cyanide ions also react with Ninhydrin to form various chromophores of analytical interest. Since its discovery, extensive efforts have been made to apply manual and automated Ninhydrin reactions as well as Ninhydrin spray reagents to the detection, isolation, and analysis of numerous compounds of interest across a broad spectrum of disciplines. These include agricultural, biochemical, clinical, environmental, food, forensic, histochemical, microbiological, medical, nutritional, plant, and protein sciences. This reaction is unique among chromogenic reactions in that at pH 5.5 it results in the formation of the same soluble chromophore by all primary amines which react, be they amines, amino acids, peptides, proteins, and even ammonia. Because the chromophore is not chemically bound to the protein or other insoluble material, it is not lost when the insoluble substrate is removed by centrifugation or filtration after the reaction is completed. The visible color of the chromophore is distinctive and is generally not affected by the yellow colors present in many food, plant, and tissue extracts. Adaptations of the classical Ninhydrin reaction to specialized needs in analytical chemistry and biochemistry include the use of acid, alkaline, and fluorogenic Ninhydrin reagents. To cross-fertilize information among several disciplines wherein an interest in the Ninhydrin reaction has developed, and to enhance its utility, this review attempts to integrate and correlate the widely scattered literature on Ninhydrin reactions of a variety of structurally different compounds. Specifically covered are the following aspects: historical perspective, chemistry and mechanisms, applications, and research needs. A better understanding of these multifaceted Ninhydrin reactions provide a scientific basis for further improvements of this important analytical technique.
A convenient Ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent
Anal Biochem 2022 Oct 1;654:114819.PMID:35839914DOI:10.1016/j.ab.2022.114819.
An improved and convenient Ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.