NKL 22
(Synonyms: Histone Deacetylase Inhibitor IV) 目录号 : GC32964
A selective HDAC1/3 inhibitor
Cas No.:537034-15-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
PAOA is a selective histone deacetylase (HDAC) inhibitor with IC50 values of 199 and 69 nM for HDAC1 and HDAC3, respectively.1 It is selective for HDAC1 and HDAC3, having IC50 values greater than 1.59 μM for HDAC2, 4, 5, 7, and 8 in a cell-free enzyme assay. In vitro, PAOA induces histone H3 and H4 hyperacetylation.2 PAOA improves the metabolic deficit exhibited by murine striatal cells isolated from HdhQ111 knock-in mice in a dose-dependent manner and reduces eye neurodegeneration in a D. melanogaster model of Huntington’s disease.1 In vivo, PAOA prevents formation of Huntingtin (Htt) protein aggregates in the brain and reduces the cognitive deficits in the N171-82Q mouse model of Huntington’s disease.3
1.Jia, H., Pallos, J., Jacques, V., et al.Histone deacetylase (HDAC) inhibitors targeting HDAC3 and HDAC1 ameliorate polyglutamine-elicited phenotypes in model systems of Huntington's diseaseNeurobiol. Dis.46(2)351-361(2012) 2.Mai, A., Perrone, A., Nebbioso, A., et al.Novel uracil-based 2-aminoanilide and 2-aminoanilide-like derivatives: Histone deacetylase inhibition and in-cell activitiesBioorg. Med. Chem. Lett.18(8)2530-2535(2008) 3.Jia, H., Kast, R.J., Steffan, J.S., et al.Selective histone deacetylase (HDAC) inhibition imparts beneficial effects in Huntington's disease mice: Implications for the ubiquitin-proteasomal and autophagy systemsHum. Mol. Genet.21(24)5280-5293(2012)
| Cas No. | 537034-15-4 | SDF | |
| 别名 | Histone Deacetylase Inhibitor IV | ||
| Canonical SMILES | O=C(NC1=CC=CC=C1N)CCCCCC(NC2=CC=CC=C2)=O | ||
| 分子式 | C19H23N3O2 | 分子量 | 325.4 |
| 溶解度 | DMSO : ≥ 29 mg/mL (89.12 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0731 mL | 15.3657 mL | 30.7314 mL |
| 5 mM | 0.6146 mL | 3.0731 mL | 6.1463 mL |
| 10 mM | 0.3073 mL | 1.5366 mL | 3.0731 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。