Norbergenin
(Synonyms: Norbergenin; Demethylbergenin,) 目录号 : GC19765
Norbergenin 是 bergenin 的 O-去甲基衍生物，具有中等的抗氧化活性（在 DPPH 自由基清除中 IC50 为 13 μM；在超氧阴离子清除中为 32 μM）。
Cas No.:79595-97-4
Sample solution is provided at 25 µL, 10mM.
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Norbergenin, an O-demethylated derivative of bergenin, can be isolated from rhizomes of Bergenia stacheyi.
Norbergenin shows anti-arthritic activity through modulation of Th1/Th2 cytokine balance, and it inhibits the production of proinflammatory Th1 cytokines (IL-2, IFN-γ and TNF-α) while as potentiate anti-inflammatory Th2 cytokines (IL-4 and IL-5) in the peripheral blood of adjuvant-induced arthritic balb/c mice.[1]
Norbergenin inhibits bovine adrenal tyrosine hydroxylase (TH) activity by 29.0% and 53.4% at a concentration of 20 μg/mL, and exhibited noncompetitive inhibition of TH activity with the substrate L-tyrosine. [2]
Norbergenin shows moderate antioxidant activity (IC50 13 μM in DPPH radical scavenging; 32 μM in superoxide anion scavenging). [3]
Reference:
[[1] Nazir N, et al. J Ethnopharmacol. 2007 Jun 13;112(2):401-5.
[2] Zhang YH, et al. Phytother. Res. 2003 Sep;17(8):967-9.
[3] Takahashi H, et al. Bioorg. Med. Chem. 2003 Apr 17;11(8):1781-8.
| Kinase experiment [1]: | |
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Inhibition of TH activity: Norbergenin inhibits the bovine adrenal TH activity by 51.0% at 5 μg/mL, 51.6% at 10 μg/mL, 53.4% at 20 μg/mL, respectively. |
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| Cell experiment [2]: | |
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Cell lines |
Neuronal cells (Rat) |
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Preparation method |
The solubility of this compound in DMSO is > 1 mM.. |
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Reaction Conditions |
3 days |
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Applications |
Norbergenin can enhance antioxidant activity, which can be further related to protecting neurons from damage by ROS |
| Animal experiment [3]: | |
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Animal models |
balb/c mice |
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Dosage form |
5, 10, 20, 40 and 80 mg/kg per oral dose |
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Applications |
Norbergenin inhibit Th1 (IL-2, IFN-γ and TNF-α) and up-regulates Th2 cytokines (IL-4 and IL-5) significantly, and release the symptom of oedema at 40 and 80 mg/kg. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Nazir N, et al. J Ethnopharmacol. 2007 Jun 13;112(2):401-5. [2] Zhang YH, et al. Phytother. Res. 2003 Sep;17(8):967-9. [3] Takahashi H, et al. Bioorg. Med. Chem. 2003 Apr 17;11(8):1781-8. |
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| Cas No. | 79595-97-4 | SDF | |
| 别名 | Norbergenin; Demethylbergenin, | ||
| 化学名 | Pyrano(3,2-c)(2)benzopyran-6(2H)-one, 3,4,4a,10b-tetrahydro-3,4,8,9,10-pentahydroxy-2-(hydroxymethyl)-, (2R-(2alpha,3beta,4alpha,4aalpha,10bbeta))- | ||
| Canonical SMILES | O=C1C2=CC(O)=C(O)C(O)=C2[C@@]3([H])[C@]([C@@H](O)[C@H](O)[C@@H](CO)O3)([H])O1 | ||
| 分子式 | C13H14O9 | 分子量 | 314.246 |
| 溶解度 | DMSO : 125 mg/mL (397.79 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1822 mL | 15.9111 mL | 31.8222 mL |
| 5 mM | 0.6364 mL | 3.1822 mL | 6.3644 mL |
| 10 mM | 0.3182 mL | 1.5911 mL | 3.1822 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet