Norfloxacin-d5
(Synonyms: 诺氟沙星 d5) 目录号 : GC47796
An internal standard for the quantification of norfloxacin
Cas No.:1015856-57-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Norfloxacin-d5 is intended for use as an internal standard for the quantification of norfloxacin by GC- or LC-MS. Norfloxacin is a fluoroquinolone antibiotic that inhibits the growth of Gram-positive and Gram-negative bacteria (MICs = 4 and 1 μg/ml for S. aureus and P. aeruginosa, respectively).1 It also inhibits the growth S. pseudintermedius, S. aureus, E. coli, Pasturella, and S. canis isolates from dogs (mean MIC50s = 0.25, 1, 0.03, 1, and 1 μg/ml, respectively).2 Topical administration of norfloxacin (0.1% v/v) reduces corneal ulcer size in a rabbit model of P. aeruginosa corneal infection. It also prevents encrusted cystitis in bladder and increases survival in a rat model of Corynebacterium group D2 infection when administered at a dose of 80 mg/kg per day.3 Formulations containing norfloxacin have been used to treat urinary tract and gynecological infections.
1.Darrell, R.W., Modak, S.M., and Fox, C.L., Jr.Norfloxacin and silver norfloxacin in the treatment of Pseudomonas corneal ulcer in the rabbit.Trans. Am. Opthalmol. Soc.8275-91(1984) 2.Awji, E.G., Damte, D., Lee, S.-J., et al.The in vitro activity of 15 antimicrobial agents against bacterial isolates from dogsJ. Vet. Med. Sci.74(8)1091-1094(2012) 3.Soriano, F., Rodriguez-Tudela, J.L., Castilla, C., et al.Treatment of encrusted cystitis caused by Corynebacterium group D2 with norfloxacin, ciprofloxacin, and teicoplanin in an experimental model in ratsAntimicrob. Agents Chemother.35(12)2587-2590(1991)
| Cas No. | 1015856-57-1 | SDF | |
| 别名 | 诺氟沙星 d5 | ||
| Canonical SMILES | O=C(O)C1=CN(C([2H])([2H])C([2H])([2H])[2H])C2=CC(N3CCNCC3)=C(F)C=C2C1=O | ||
| 分子式 | C16H13D5FN3O3 | 分子量 | 324.4 |
| 溶解度 | DMSO : 5 mg/mL (15.41 mM; Need ultrasonic); H2O : 1 mg/mL (3.08 mM; ultrasonic and warming and heat to 80°C) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0826 mL | 15.4131 mL | 30.8261 mL |
| 5 mM | 0.6165 mL | 3.0826 mL | 6.1652 mL |
| 10 mM | 0.3083 mL | 1.5413 mL | 3.0826 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Development of a quantitative multiclass/multiresidue method for 21 veterinary drugs in shrimp
J AOAC Int 2011 Mar-Apr;94(2):394-406.PMID:21563672doi
A multiclass/multiresidue method has been developed and validated for the determination of 21 veterinary drug residues in shrimp, including sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine, sulfachloropyridazine, sulfadimethoxine, and sulfaquinoxaline); tetracyclines (oxytetracycline, tetracycline, and chlortetracycline); (fluoro)quinolones (norfloxacin, ciprofloxacin, enrofloxacin, sarafloxacin, difloxacin, flumequine, oxolinic acid, and nalidixic acid); and cationic dyes (malachite green, gentian violet, leucomalachite green, and leucogentian violet), using HPLC/MS/MS. All drugs were quantifiable over a no less than 10-fold range with matrix-matched standards for linear external calibration, except for oxytetracycline, tetracycline, norfloxacin, and ciprofloxacin, for which Norfloxacin-d5 was used as an internal standard. Two grams of preground shrimp sample was extracted twice with extractant at two different pH values. The combined supernatant was further diluted with an aqueous internal standard solution, and 50 microL extract was injected into the HPLC instrument. An online SPE system was set up for automated sample cleanup. A triple quadrupole mass spectrometer equipped with an electrospray ionization source was operated in the multiple-reaction-monitoring mode to acquire data. The method has been validated at three levels within the designated linear ranges for each drug, with accuracies between 77 and 115%, and most CV values below 15%.