O4I1
(Synonyms: 2-(4-[(4-甲氧基苄基)氧基]苯基)乙腈) 目录号 : GC12499An inducer of Oct3/4
Cas No.:175135-47-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
O4I1 is a potent Oct3/4 inducer implicated in promoting the expression and stabilization of Oct3/4. O4I1 can enhance the transcriptional activity of Oct3/4 in a variety of human somatic cells. Oct3/4, also known as POU5F1 and Oct4, is a transcription factor which is essential for self-renewal and pluripotency of embryonic stem cells. Oct3/4 is specifically expressed in the ES cells, epiblast, preimplantation embryos, and germ cells, et al [1].
When treated the synthesized cDNA from RNA isolated from the HEK293 cells with 10 µM and 20 µM O4I1 for 72 hrs, the Oct3/4 mRNA levels increased about 2.5- and 4-fold respectively. In human adult primary fibroblast (HF) and human neonatal foreskin fibroblast (HFF) cell lines, treatment with 10 µM O4I1 increased the gene and protein expression level of Oct3/4 after 72 hrs incubation in HF cells and 7 days in HFF cells. The derivatives of O4I1 such as compound 2, showed a time-dependent activation of Oct3/4 and markedly elevated the efficiency of Oct3/4 induction [1].
Reference:
[1].Cheng X, Dimou E, Alborzinia H, et al. Identification of 2-[4-[(4-Methoxyphenyl) methoxy]-phenyl] acetonitrile and Derivatives as Potent Oct3/4 Inducers[J]. Journal of medicinal chemistry, 2015, 58(12): 4976-4983.
Cas No. | 175135-47-4 | SDF | |
别名 | 2-(4-[(4-甲氧基苄基)氧基]苯基)乙腈 | ||
化学名 | 2-(4-((4-methoxybenzyl)oxy)phenyl)acetonitrile | ||
Canonical SMILES | COC1=CC=C(COC2=CC=C(CC#N)C=C2)C=C1 | ||
分子式 | C16H15NO2 | 分子量 | 253.3 |
溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2)(1:5): 0.16 mg/ml,Ethanol: slightly | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.9479 mL | 19.7394 mL | 39.4789 mL |
5 mM | 0.7896 mL | 3.9479 mL | 7.8958 mL |
10 mM | 0.3948 mL | 1.9739 mL | 3.9479 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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