OAC1
(Synonyms: N-1H-吡咯并[2,3-C]吡啶-5-基苯甲酰胺) 目录号 : GC10151
An Oct4-activating compound
Cas No.:300586-90-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
OAC1 is an activator of Oct4 [1].
Octamer-binding transcription factor 4 (Oct4) is a regulator of embryonic stem cell (ESC) pluripotency and the reprogramming process. High expression of Oct4 results in differentiation of ESCs along the mesodermal and primitive endodermal lineages. However, low expression of Oct4 leads to differentiation of ESCs into trophectodermal cells [1].
OAC1 is an Oct4 activator. In luciferase reporter assays, OAC1 activated both human Oct4 and Nanog promoter-driven luciferase reporter genes. In mouse embryonic fibroblasts (MEFs) with the 4F reprogramming quartet Oct4, Klf4, Sox2, and c-Myc, OAC1 enhanced reprogramming efficiency and increased the number of colonies with ESC-like morphology by twofold or more. Also, OAC1 increased induced pluripotent stem cells (iPSC)-like colonies. OAC1 had no effects on the Wnt signaling and p53-p21 expression. In MEFs, OAC1 activated the expression of endogenous Oct4 and Nanog. Also, OAC1 increased the expression of Sox2 and Tet1 [1].
Reference:
[1]. Li W, Tian E, Chen ZX, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A, 2012, 109(51): 20853-20858.
| Cas No. | 300586-90-7 | SDF | |
| 别名 | N-1H-吡咯并[2,3-C]吡啶-5-基苯甲酰胺 | ||
| 化学名 | N-(1H-pyrrolo[2,3-c]pyridin-5-yl)benzamide | ||
| Canonical SMILES | C1=CC=C(C=C1)C(=O)NC2=NC=C3C(=C2)C=CN3 | ||
| 分子式 | C14H11N3O | 分子量 | 237.26 |
| 溶解度 | ≥ 9.9mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.2148 mL | 21.0739 mL | 42.1479 mL |
| 5 mM | 0.843 mL | 4.2148 mL | 8.4296 mL |
| 10 mM | 0.4215 mL | 2.1074 mL | 4.2148 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。