Octadecyl Rhodamine B chloride
(Synonyms: 十八基罗丹明B氯化物) 目录号 : GC64502
Octadecyl Rhodamine B chloride 是一种阳离子两亲物，可用于细胞膜染色。可用于多种研究，包括在有机分子组装、膜结构及蛋白质结构域中的电子能量转移。
Cas No.:65603-19-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Octadecyl Rhodamine B chloride is a cationic amphiphile that can be used for staining cell membranes. Octadecyl Rhodamine B chloride can be used in numerous studies including electronic energy transfer in organized molecular assemblies, membrane structure, and distances of closest approach between protein domains and membranes[1].
[1]. Tione Buranda, et al. Real-time partitioning of octadecyl rhodamine B into bead-supported lipid bilayer membranes revealing quantitative differences in saturable binding sites in DOPC and 1:1:1 DOPC/SM/cholesterol membranes. J Phys Chem B. 2010 Jan 28;114
| Cas No. | 65603-19-2 | SDF | Download SDF |
| 别名 | 十八基罗丹明B氯化物 | ||
| 分子式 | C46H67ClN2O3 | 分子量 | 731.49 |
| 溶解度 | DMSO : 125 mg/mL (170.88 mM; Need ultrasonic) | 储存条件 | 4°C, away from moisture |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3671 mL | 6.8354 mL | 13.6707 mL |
| 5 mM | 0.2734 mL | 1.3671 mL | 2.7341 mL |
| 10 mM | 0.1367 mL | 0.6835 mL | 1.3671 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quenching and dequenching of Octadecyl Rhodamine B chloride fluorescence in Ca(2+)-induced fusion of phosphatidylserine vesicles: effects of poly(ethylene glycol)
Biochim Biophys Acta 1994 May 11;1191(2):375-83.PMID:8172923DOI:10.1016/0005-2736(94)90189-9.
Ca(2+)-induced fusion of SUV and LUV composed of ox brain phosphatidylserine (PS) was studied as a function of temperature and concentration of Ca2+ using Octadecyl Rhodamine B chloride (R-18). Ca2+ was added to a 1:1 mixture of labelled (8 mol%) and unlabelled vesicles (assay conditions) or to samples containing only labelled liposomes (control conditions). Both, in SUV and LUV the dependence of differences in fluorescence between assay and control samples on temperature can be divided into three regions. At temperatures lower than 20 degrees C the differences in fluorescence increase only slightly in SUV or remain unchanged in LUV after the addition of Ca2+. At 28 degrees C and higher temperatures the differences of fluorescence intensities increase much more drastically, whereby SUV exhibit higher fusion rates than LUV. Between 20 degrees C and 28 degrees C exists an intermediate region for both SUV and LUV. Here the fluorescence changes continuously from one behaviour to the other independent of the concentration of Ca2+. A drastic quenching of R-18 fluorescence occurs in LUV composed of PS below 10 degrees C, where the lipids are in the gel state. In SUV the fluorescence is only weakly changed in this temperature region. It is assumed that a demixing between dye and phospholipid molecules occurs below phase transition. During fusion the phase transition of PS is shifted from 8-10 degrees C to about 24-28 degrees C as revealed by polarization measurements using diphenylhexatriene. Because the differences in R-18 fluorescence between assay and control samples depend strongly on temperature we assume that the shift in phase transition temperature of PS occurs immediately after the addition of Ca2+ to SUV or LUV. Poly(ethylene glycol) 6000 accelerates fusion in both SUV and LUV under all conditions where a fusion takes place. Further, the threshold concentration of Ca2+ to induce fusion is diminished from about 1 mmol/l without polymers to about 0.5 mmol/l in the presence of 10% (w/v) PEG 6000. The intermediate region of changes in fluorescence properties of R-18 in the Ca(2+)-induced fusion of PS is not changed by PEG.
Fusion between Newcastle disease virus and erythrocyte ghosts using octadecyl Rhodamine B fluorescence assay produces dequenching curves that fit the sum of two exponentials
Biochem J 1994 Jun 1;300 ( Pt 2)(Pt 2):347-54.PMID:8002938DOI:10.1042/bj3000347.
The kinetics of fusion between Newcastle disease virus and erythrocyte ghosts has been investigated with the Octadecyl Rhodamine B chloride assay [Hoekstra, De Boer, Klappe, and Wilschut (1984) Biochemistry 23, 5675-5681], and the data from the dequenching curves were fitted by non-linear regression to currently used kinetic models. We used direct computer-assisted fitting of the dequenching curves to the mathematical equations. Discrimination between models was performed by statistical analysis of different fits. The experimental data fit the exponential model previously published [Nir, Klappe, and Hoekstra (1986) Biochemistry 25, 2155-2161] but we describe for the first time that the best fit was achieved for the sum of two exponential terms: A1[1-exp(-k1t)]+A2[1-exp(-k2t)]. The first exponential term represents a fast reaction and the second a slow dequenching reaction. These findings reveal the existence of two independent, but simultaneous, processes during the fusion assay. In order to challenge the model and to understand the meaning of both equation, fusion experiments were carried out under different conditions well known to affect viral fusion (changes in pH, temperature and ghost concentration, and the presence of disulphide-reducing agents or inhibitors of viral neuraminidase activity), and the same computer fitting scheme was followed. The first exponential equation represents the viral protein-dependent fusion process itself, because it is affected by the assay conditions. The second exponential equation accounts for a nonspecific reaction, because it is completely independent of the assay conditions and hence of the viral proteins. An interpretation of this second process is discussed in terms of probe transfer between vesicles.
Dynamics of exosome internalization and trafficking
J Cell Physiol 2013 Jul;228(7):1487-95.PMID:23254476DOI:10.1002/jcp.24304.
Cells release exosomes into extracellular medium. Although the important roles of exosomes in many physiological and pathological processes are being revealed, the mechanism of exosome-cell interaction remains unclear. In this article, employing real-time fluorescence microscopy, the motion of exosomes on the plasma membrane or in the cytoplasm of recipient PC12 cells was observed directly. In addition, several motion modes of exosomes were revealed by single particle tracking (SPT). The changes between motion modes were also detected, presenting the dynamic courses of exosome attachment onto plasma membrane and exosome uptake. Octadecyl Rhodamine B chloride (R18) was found to be useful to distinguish endocytosis from fusion during exosome uptake. Colocalization with organelle markers showed exosomes were sorted to acidic vesicles after internalization. The results provide new sight into the exosome-cell interaction mode and the intercellular trafficking of exosomes. This study will help to understand the roles of exosomes at cell level.
Langmuir-Blodgett monolayers of cationic dyes in the presence and absence of clay mineral layers: N,N'-dioctadecyl thiacyanine, octadecyl rhodamine B and laponite
Langmuir 2010 Jul 20;26(14):11870-7.PMID:20521840DOI:10.1021/la101078f.
Langmuir-Blodgett (LB) films of N,N'-dioctadecyl thiacyanine perchlorate (TC18) and Octadecyl Rhodamine B chloride (RhB18) and their mixtures in the presence and absence of clay mineral layers were investigated by recording surface pressure-area (pi-A) isotherms and by UV-vis and fluorescence spectroscopies. The pi-A isotherms of TC18, RhB18, and their mixtures are characteristic of liquid expanded state behavior with repulsive interactions between the two cationic dyes. In the presence of laponite, the pi-A isotherms show liquid expanded and condensed-state behavior. In laponite dispersions and in monolayers, TC18 has a strong tendency to aggregate with formation of H- and J- aggregates. The absorption and fluorescence maxima of the monomers in the films are at 435 nm and at 480 nm; H-dimers have an absorption maximum around 410 nm and do not fluoresce. J-dimers are present in all the films with absorption maximum at 461 nm and fluorescence at 463 nm. RhB18 is mainly present as monomers in the LB films with an absorption maximum at 576 nm and fluorescence at 595 nm. Fluorescence resonance energy transfer from TC18 to RhB18 has been observed in clay dispersions and in films with and without laponite. The optimum condition for TC18 --> RhB18 fluorescence energy transfer in the films is 90 mol % TC18 + 10 mol % RhB18.
Epstein-Barr virus enters B cells and epithelial cells by different routes
J Virol 1992 Jun;66(6):3409-14.PMID:1316456DOI:10.1128/JVI.66.6.3409-3414.1992.
Epstein-Barr virus (EBV) infects two cell types, B lymphocytes and epithelial cells. Electron microscopic studies have shown that the virus fuses with the lymphoblastoid cell line Raji but is endocytosed into thin-walled non-clathrin-coated vesicles in normal B cells before fusion takes place. To compare early interactions of EBV with epithelial cells and B cells, a fluorescence dequenching assay of fusion was employed, using virus labeled either with the pH-insensitive probe Octadecyl Rhodamine B chloride (R18) or with 5(N-octadecanoyl) aminofluorescein (AF), which loses emission intensity at a pH below 7.4. Fusion of virus labeled with R18 could be monitored with B cells, Raji cells, and epithelial cells. Lowering the extracellular pH or pretreatment of cells with ammonium chloride or methylamine had no effect on these measurements. In contrast, fusion of virus labeled with AF could be measured with Raji cells and epithelial cells, but not with normal B cells unless cells were previously treated with ammonium chloride. Fusion of virus with normal B cells was inhibited with chlorpromazine, chloroquine, and sodium azide, but none of these reagents had any effect on fusion with Raji or epithelial cells. These results suggest that entry of EBV into nonpolarized suspensions of epithelial cells occurs by fusion at the cell surface, that EBV may be incapable of fusing with normal B cells unless it has first been endocytosed, and that pH appears to be irrelevant to either event. A combination of the two probes, R18 and AF, may have general use for determining the sites of entry of enveloped viruses that fuse in a pH-independent manner.