Oil Red O
(Synonyms: ORO) 目录号 : GC25676油红O是一种红色中性脂滴染色剂。
Cas No.:1320-06-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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1. Oil Red O染色准备 1.1 Oil Red O原液：将0.5gOil Red O溶于200ml异丙醇中，56℃反应1h。 进一步使用前请先冷却。 室温保存。 1.2 Oil Red O工作液：使用当天新鲜配制。 将四份蒸馏水与六份储备溶液混合。 让溶液静置至少 15 分钟。 将形成沉淀。 通过滤纸过滤溶液，例如3MM Chr Whatman滤纸。 使用前保持室温。 1.3 1×PBS，pH 7.4。 1.4 60% (v/v) 异丙醇的蒸馏水溶液。 2. 固定细胞 2.1 用 PBS 仔细清洗细胞，因为它们很容易从板上脱落。 2.2 每孔加入 1 ml 4% PFA，在水平表面室温孵育 20 分钟，不要摇动或倾斜。 2.3 将 PFA 丢弃在危险废物室中，并在 PBS 中洗涤细胞两次，每次 5 分钟。 3. 盖玻片的安装 3.1 在载玻片中间滴加 40 ml 封固液。 3.2 用镊子从六孔板上取下盖玻片，并用纸巾吸干边缘。 然后将盖玻片放在封固液上（细胞面朝下），注意不要留有气泡。 3.3 室温避光孵育2小时，使封固液凝固。 3.4 用湿纸巾擦去盖玻片上的沉淀物。 3.5 将载玻片置于4℃避光保存。 4. Oil Red O染色 4.1 固定细胞并去除 PBS（参见第 2 节）。 4.2 用60%异丙醇洗涤细胞。 4.3 每孔加入 1.5 ml Oil Red O 工作液，孵育 15-30 分钟。 在显微镜下观察，直至细胞正确染色。 当/如果沉淀开始形成时，请务必停止染色。 用 60% 异丙醇清洗细胞。 4.4 用 1 ml PBS 洗涤细胞并保留在 PBS 中直至进一步使用。 4.5 将盖玻片安装在载玻片上或使用染色细胞通过Oil Red O 提取进行定量。 Oil Red O 染色的细胞可以直接拍照，或者可以使用任何配备 RGB 相机的显微镜获得盖玻片上细胞的更高放大倍数图像。 |
Oil Red O is a type of lysochrome diazo dye, also known as Solvent Red 27, Sudan Red 5B. It is commonly used for staining neutral triglycerides and lipids on frozen sections, as well as certain lipoproteins on paraffin sections. Oil Red O is in the form of a red powder and exhibits a peak absorbance at 518 nm [1].
Reference:
[1]. Kraus NA, Ehebauer F, Zapp B, Rudolphi B, Kraus BJ, Kraus D. Quantitative assessment of adipocyte differentiation in cell culture. Adipocyte. 2016 Oct 1;5(4):351-8.
Oil Red O（红油O,溶剂红27号，苏丹红5B）是一种脂溶性染料，用于在冰冻切片上染色中性甘油三酯和脂质，以及在石蜡切片上染色某些脂蛋白。它呈现为红色粉末，吸收峰位于518nm[1]。
| Cas No. | 1320-06-5 | SDF | Download SDF |
| 别名 | ORO | ||
| 分子式 | C26H24N4O | 分子量 | 408.49 |
| 溶解度 | DMSO : 4.17 mg/mL (10.21 mM; ultrasonic and warming and heat to 80°C) | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.448 mL | 12.2402 mL | 24.4804 mL |
| 5 mM | 0.4896 mL | 2.448 mL | 4.8961 mL |
| 10 mM | 0.2448 mL | 1.224 mL | 2.448 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Methods Mol Biol 2015;1339:85-99.PMID:26445782DOI:10.1007/978-1-4939-2929-0_5.
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
Imaging of neutral lipids by Oil Red O for analyzing the metabolic status in health and disease
Nat Protoc 2013 Jun;8(6):1149-54.PMID:23702831DOI:10.1038/nprot.2013.055.
Excess lipid accumulation in peripheral tissues is a key feature of many metabolic diseases. Therefore, techniques for imaging and quantifying lipids in various tissues are important for understanding and evaluating the overall metabolic status of a research subject. Here we present a protocol that detects neutral lipids and lipid droplet (LD) morphology by Oil Red O (ORO) staining of sections from frozen tissues. The method allows for easy estimation of tissue lipid content and distribution using only basic laboratory and computer equipment. Furthermore, the procedure described here is well suited for the comparison of different metabolically challenged animal models. As an example, we include data on muscular and hepatic lipid accumulation in diet-induced and genetically induced diabetic mice. The experimental description presents details for optimal staining of lipids using ORO, including tissue collection, sectioning, staining, imaging and measurements of tissue lipids, in a time frame of less than 2 d.
Oil Red O Staining for Lipid Content in Caenorhabditis elegans
Bio Protoc 2021 Aug 20;11(16):e4124.PMID:34541042DOI:10.21769/BioProtoc.4124.
The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an optimized protocol for ORO staining of C. elegans and provide detailed instructions for quantifying the intensity of ORO signal in images acquired by light microscopy.
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
J Vis Exp 2018 Mar 5;(133):57352.PMID:29553519DOI:10.3791/57352.
Caenorhabditis elegans is an exceptional model organism in which to study lipid metabolism and energy homeostasis. Many of its lipid genes are conserved in humans and are associated with metabolic syndrome or other diseases. Examination of lipid accumulation in this organism can be carried out by fixative dyes or label-free methods. Fixative stains like Nile red and Oil Red O are inexpensive, reliable ways to quantitatively measure lipid levels and to qualitatively observe lipid distribution across tissues, respectively. Moreover, these stains allow for high-throughput screening of various lipid metabolism genes and pathways. Additionally, their hydrophobic nature facilitates lipid solubility, reduces interaction with surrounding tissues, and prevents dissociation into the solvent. Though these methods are effective at examining general lipid content, they do not provide detailed information about the chemical composition and diversity of lipid deposits. For these purposes, label-free methods such as GC-MS and CARS microscopy are better suited, their costs notwithstanding.
Oil Red O based method for exosome labelling and detection
Biochem Biophys Res Commun 2022 Jun 30;611:179-182.PMID:35490657DOI:10.1016/j.bbrc.2022.04.087.
With the realization of the role of exosomes in diseases, especially cancer, exosome research is gaining popularity in biomedical sciences. To understand exosome biology, their labelling and tracking studies are important. New and improved methods of exosome labelling for detection and tracking of exosomes need to be developed to harness their therapeutic and diagnostic potential. In this paper, we report a novel, simple and effective method of labelling and detecting exosomes using Oil Red O (ORO), a dye commonly used for lipid staining. Using ORO is a cost effective and easy approach with an intense red coloration of exosomes. Further, the issues faced with commonly used lipophilic dyes for exosome labelling like long-term persistence of dyes, aggregation and micelle formation of dyes, difficulty in distinguishing dye particles from labelled exosomes, and detection of large aggregates of dye or dye-exosome, are also resolved with ORO dye. This method shows good labelling efficacy with very sensitive detection and real-time tracking of the cellular uptake of exosomes.