Oleic Acid-13C5
(Synonyms: 9Z-Octadecenoic Acid-13C5, C18:1(9Z)-13C5, cis-9-Octadecenoic Acid-13C5) 目录号 : GC52318An internal standard for the quantification of oleic acid
Cas No.:1255644-48-4
Sample solution is provided at 25 µL, 10mM.
Oleic acid-13C5 is intended for use as an internal standard for the quantification of oleic acid by GC- or LC-MS. Oleic acid is a monounsaturated fatty acid and a major component of membrane phospholipids that has been found in human plasma, cell membranes, and adipose tissue.1,2 It contributes approximately 17% of the total fatty acids esterified to phosphatidylcholine, the major phospholipid class in porcine platelets.1 Oleic acid inhibits collagen-stimulated platelet aggregation by approximately 90% when used at a concentration of 10 µg/ml. It also inhibits fMLF-induced neutrophil aggregation and degranulation by 55 and 68%, respectively, when used at a concentration of 5 µM, similar to arachidonic acid .3 Oleic acid (60 µM) induces release of intracellular calcium in human platelets.4 In vivo, oleic acid increases TNF-α, IL-8, IL-6, and IL-1β production, neutrophil accumulation, and apoptotic and necrotic cell death in mouse lung and has been used to induce lung injury in a mouse model of acute respiratory distress syndrome (ARDS).2
1.Wahle, K.W., and Peacock, L.I.Effects of isomeric cis and trans eighteen carbon monounsaturated fatty acids on porcine platelet functionBiochim. Biophys. Acta1301(1-2)141-149(1996) 2.GonÇalves-de-Albuquerque, C.F., Silva, A.R., Burth, P., et al.Acute respiratory distress syndrome: Role of oleic acid-triggered lung injury and inflammationMediators Inflamm.260465(2015) 3.Naccache, P.H., Moiski, T.F., Volpi, M., et al.Modulation of rabbit neutrophil aggregation and degranulation by free fatty acidsJ. Leukoc. Biol.36(3)333-340(1984) 4.Siafaka-Kapadai, A., Hanahan, D.J., and Javors, M.A.Oleic acid-induced Ca2+ mobilization in human platelets: Is oleic acid an intracellular messenger•J. Lipid Mediat. Cell Signal15(3)215-232(1997)
Cas No. | 1255644-48-4 | SDF | Download SDF |
别名 | 9Z-Octadecenoic Acid-13C5, C18:1(9Z)-13C5, cis-9-Octadecenoic Acid-13C5 | ||
Canonical SMILES | CCCCCCCC/C=C\[13CH2][13CH2]CCC[13CH2][13CH2][13C](O)=O | ||
分子式 | C13[13C]5H34O2 | 分子量 | 287.4 |
溶解度 | Chloroform: soluble,DMF: >100mg/mL,DMSO: >100mg/mL,Ethanol: >100mg/mL,PBS (pH 7.2): <100ug/mL | 储存条件 | -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.4795 mL | 17.3974 mL | 34.7947 mL |
5 mM | 0.6959 mL | 3.4795 mL | 6.9589 mL |
10 mM | 0.3479 mL | 1.7397 mL | 3.4795 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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