Optovin
目录号 : GC12597
A photoactivatable TRPA1 ligand
Cas No.:348575-88-2
Sample solution is provided at 25 µL, 10mM.
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Optovin is an activator of TRPA1 [1].
Transient receptor potential cation channel, subfamily A, member 1 (TRPA1) is an ion channel that involved in signal transduction and growth control. TRPA1 signaling plays an important role in chronic inflammation and neuropathic pain [1].
Optovin is a photoactivator of TRPA1. In dorsal root ganglia (DRG) sensory neurons isolated from mice, optovin significantly activated 33% (35/105) of DRG neurons, which was not occured in the dark. In HEK293T cells transfected with the human TRPA1, optovin activated cells. Optovin activated TRPA1 channels through a photoactivated intermediate that reacted with redox-sensitive cysteine residues in the channel [1].
In wild-type zebrafish embryos, optovin increased light-dependent motor activity with EC50 value of 2 μM. Optovin induced zebrafish respond to 387-nm light stimuli. Optovin acted on sensory neurons and was not dependent on visual pathways for the increase of light-dependent motor activity. In zebrafish with trpa1b mutant, optovin didn’t have effect, which suggested trpa1b is necessary for optovin activity. In adult mice, optovin induced head-shaking behaviors [1].
Reference:
[1]. Kokel D, Cheung CY, Mills R, et al. Photochemical activation of TRPA1 channels in neurons and animals. Nat Chem Biol, 2013, 9(4): 257-263.
| Cas No. | 348575-88-2 | SDF | |
| 化学名 | (E)-5-((2,5-dimethyl-1-(pyridin-3-yl)-1H-pyrrol-3-yl)methylene)-4-hydroxythiazole-2(5H)-thione | ||
| Canonical SMILES | CC(N1C2=CN=CC=C2)=CC(/C([H])=C(S3)\C(O)=NC3=S)=C1C | ||
| 分子式 | C15H13N3OS2 | 分子量 | 315.41 |
| 溶解度 | DMF: 25 mg/ml,DMF:PBS(pH 7.2)(1:6): 0.14 mg/ml,DMSO: 20 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1705 mL | 15.8524 mL | 31.7048 mL |
| 5 mM | 0.6341 mL | 3.1705 mL | 6.341 mL |
| 10 mM | 0.317 mL | 1.5852 mL | 3.1705 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet