Orexin B (human)
(Synonyms: 食欲肽B(人),Human orexin B) 目录号 : GC14887
Orexin B (human)是一种由28个氨基酸残基组成的肽，对OX1和OX2受体具有亲和力，Ki值分别为420nM和 3nM。
Cas No.:205640-91-1
Sample solution is provided at 25 µL, 10mM.
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Orexin B (human), a 28-residue peptides, has affinity for the OX1 and OX2 receptor with Ki of 420 and 36nM, respectively[1].
Orexin B induces depolarization in both type 1 and type 2 neurons of the PVN with dose-dependent, and the changes in membrane potential are larger in type 2 neurons than in type 1[2]. Orexin B can inhibit the uptake of fAβ in BV2 cell line, reducing the levels of p-p38, p-Akt, and PI3K[3].
Orexin B was acutely administered into the lateral ventricle of male rats through preimplanted indwelling catheters, Orexin B significantly augmented food intake; at the 2h time point, observed 5-and 12-fold stimulation of food consumption by 3nmol and 30nmol Orexin B, respectively, as compared with vehicle controls[4]. Orexin B will be rapidly degraded in blood, so no 125I-Orexin B could be detected in intact form in brain when injected peripherally in male albino ICR mice at a dose of 6pmol/mouse through the isolated left jugular vein[5].
References:
[1] Smart D. Orexins: a new family of neuropeptides[J]. British journal of anaesthesia, 1999, 83(5): 695-697.
[2] Shirasaka T, Miyahara S, Kunitake T, et al. Orexin depolarizes rat hypothalamic paraventricular nucleus neurons[J]. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2001, 281(4): R1114-R1118.
[3] An H, Cho M H, Kim D H, et al. Orexin impairs the phagocytosis and degradation of amyloid-β fibrils by microglial cells[J]. Journal of Alzheimer’s Disease, 2017, 58(1): 253-261.
[4] Sakurai T, Amemiya A, Ishii M, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior[J]. Cell, 1998, 92(4): 573-585.
[5] Kastin A J, Akerstrom V. Orexin A but not orexin B rapidly enters brain from blood by simple diffusion[J]. The Journal of pharmacology and experimental therapeutics, 1999, 289(1): 219-223.
Orexin B (human)是一种由28个氨基酸残基组成的肽，对OX1和OX2受体具有亲和力，Ki值分别为420nM和 3nM[1]。
Orexin B呈剂量依赖性地诱导室旁核（PVN）的1型和2型神经元发生去极化，并且2型神经元的膜电位变化大于1型神经元[2]。Orexin B能够抑制BV2细胞系对纤维状β淀粉样蛋白（fAβ）的摄取，降低p-p38、p-Akt和PI3K的水平[3]。
Orexin B急性注入雄性大鼠的侧脑室能够显著增加食物摄入量；在注射后2h的时间点，与对照组相比，3nmol和30nmol的Orexin B分别使食物消耗量增加了5倍和12倍[4]。Orexin B在血液中会迅速降解，每只雄性白化ICR小鼠 6pmol的剂量进行外周注射时，在小鼠大脑中无法检测到完整形式的125I标记的Orexin B[5]。
| Cell experiment [1]: | |
Cell lines | Microglial BV2 cells |
Preparation Method | Microglial BV2 cells were seeded on 12 well plates. After the cells settled, they were treated with either fibrillary Aβ (fAβ) (1μM) and Orexin A (OXA); fAβ (1μM) and Orexin B (OXB); or fAβ only. Then they were cultured for 5min, 30min, 2h, or 24h. OXA and OXB were added 30min before fAß to assure it had enough time to be absorbed by the cells prior to treatment by fAβ. Both OXA and OXB were used to investigate differences between the Orexin receptors. |
Reaction Conditions | 1μM; 30min |
Applications | Orexin A and Orexin B can suppresses the uptake of fAβ in BV2 microglial cells. |
| Animal experiment [2]: | |
Animal models | Male albino ICR mice |
Preparation Method | The 125I-Orexin A or 125I-Orexin B was injected in a dose of 6pmol/mouse (2×106cpm) through the isolated left jugular vein along with 2μCi/mouse of 99mTc-albumin in 200μl of lactated Ringer’s solution containing 1% albumin. At 2, 3, 4, 5, 6, 8, 10, 20, and 30min after i.v. injection, blood was collected from a cut in the right carotid artery, and then the mouse was immediately decapitated. Serum and brain samples were obtained and counted in a dual channel γ-counter. The ratio of the radioactivity of brain tissue and serum was calculated, and multiple time-regression analysis was applied to determine the relationship between the ratios and exposure time. |
Dosage form | 6pmol/mouse; 30min; i.v. |
Applications | No 125I-Orexin B could be detected in intact form in brain when injected peripherally in male albino ICR mice at a dose of 6pmol/mouse through the isolated left jugular vein, while most of the injected 125I-Orexin A reached the brain as intact peptide. |
References: | |
| Cas No. | 205640-91-1 | SDF | |
| 别名 | 食欲肽B(人),Human orexin B | ||
| 化学名 | (S,Z)-N'1-((5S,6Z,8S,9Z,11S,12Z,14S,15Z,18Z,20S,21Z,23S,24Z,26S,27Z,29S,30Z,33Z,35S,36Z,38S,39Z,41S,42Z,44S,45Z,47S,48Z,50S,51Z,53S,54Z,56S,57Z,59S,60Z)-26-((1H-imidazol-5-yl)methyl)-14-((S)-sec-butyl)-50,59-bis(3-guanidinopropyl)-7,10,13,16,19,22,25,28,3 | ||
| Canonical SMILES | CC[C@]([C@@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C/N=C(O)/[C@ | ||
| 分子式 | C123H212N44O35S | 分子量 | 2899.36 |
| 溶解度 | Soluble to 1 mg/ml in Water | 储存条件 | Desiccate at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3449 mL | 1.7245 mL | 3.449 mL |
| 5 mM | 0.069 mL | 0.3449 mL | 0.6898 mL |
| 10 mM | 0.0345 mL | 0.1725 mL | 0.3449 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
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- Purity: >98.00%
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