OSGEP Human
目录号 : GP22037O-Sialoglycoprotein Endopeptidase Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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OSGEP Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 358 amino acids (1-335 a.a) andhaving a molecular mass of 38.8kDa.OSGEP is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| Purity | Greater than 80.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | O-Sialoglycoprotein Endopeptidase; T(6)A37Threonylcarbamoyladenosine Biosynthesis Protein OSGEP; TRNAThreonylcarbamoyladenosine Biosynthesis Protein OSGEP; N6-L-ThreonylcarbamoyladenineSynthase; T(6)A Synthase; HOSGEP; GCPL1; Probable TRNAThreonylcarbamoyladenosine Biosynthesis Protein OSGEP; Probable TRNAN6-Adenosine Threonylcarbamoyltransferase ; Probable O-SialoglycoproteinEndopeptidase; EC 3.4.24.57; EC 2.3.1.234; FLJ20411; OSGEP1; PRSMG1; KAE1; ProbabletRNA N6-adenosine threonylcarbamoyltransferase. | ||
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMPAVLGF EGSANKIGVG VVRDGKVLAN PRRTYVTPPG TGFLPGDTARHHRAVILDLL QEALTESGLT SQDIDCIAYT KGPGMGAPLV SVAVVARTVA QLWNKPLVGV NHCIGHIEMGRLITGATSPT VLYVSGGNTQ VIAYSEHRYR IFGETIDIAVGNCLDRFARV LKISNDPSPG YNIEQMAKRG KKLVELPYTV KGMDVSFSGILSFIEDVAHR MLATGECTPE DLCFSLQETV FAMLVEITER AMAHCGSQEA LIVGGVGCNV RLQEMMATMCQERGARLFAT DERFCIDNGA MIAQAGWEMF RAGHRTPLSD SGVTQRYRTD EVEVTWRD. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | OSGEP protein solution (0.5mg/ml) containing 20mMTris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea. | ||
O-SialoglycoproteinEndopeptidase, also known as OSGEP is a member of the KAE1 / TsaD family. OSGEP is essential for the formation ofthreonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs which readcodons beginning with adenine. OSGEP take a direct catalytic part in the abovereaction, however other proteins of the complex are required to fulfill thisactivity.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.