OVA Peptide (257-264) TFA
(Synonyms: Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu, SIINFEKL, OVA (257-264)) 目录号 : GC33600
A synthetic peptide fragment of chicken ovalbumin
Cas No.:1262751-08-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide (257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1]. |
References: [1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9. | |
Ovalbumin (257-264) is a synthetic peptide fragment corresponding to the mouse MHC class I (H-2Kb) CD8+ T cell-restricted epitope of chicken ovalbumin.1,2 It is presented by H-2Kb dominant T cells and has been used to induce CD8+ T cell responses in vitro and in vivo.1,2,3
1.Wick, M.J., and Pfeifer, J.D.Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: Protein context influences the degree of TAP-independent presentationEur. J. Immunol.26(11)2790-2799(1996) 2.Chaoul, N., and Dadglio, G.Antigen-specific in vivo killing assayMethods Mol. Biol.232555-64(2021) 3.Berguer, P.M., Alzogaray, V.A., Rossi, A.H., et al.A polymeric protein induces specific cytotoxicity in a TLR4 dependent manner in the absence of adjuvantsPLoS One7(9)e45705(2012)
| Cas No. | 1262751-08-5 | SDF | |
| 别名 | Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu, SIINFEKL, OVA (257-264) | ||
| Canonical SMILES | Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu | ||
| 分子式 | C47H75F3N10O15 | 分子量 | 1077.15 |
| 溶解度 | Water : 20 mg/mL (18.57 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9284 mL | 4.6419 mL | 9.2838 mL |
| 5 mM | 0.1857 mL | 0.9284 mL | 1.8568 mL |
| 10 mM | 0.0928 mL | 0.4642 mL | 0.9284 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。