Oxythiamine (Hydroxythiamin)
(Synonyms: Hydroxythiamin) 目录号 : GC33316
Oxythiamine (Hydroxythiamin) 是一种抗代谢药和维生素 B1 拮抗剂，是众所周知的硫胺素拮抗剂和转酮酶抑制剂。
Cas No.:136-16-3
Sample solution is provided at 25 µL, 10mM.
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Oxythiamine, an antimetabolite and a vitamin B1 antagonist, is a well-known thiamine antagonist and inhibitor of transketolase.
Oxythiamine, an antimetabolite and a vitamin B1 antagonist, is a well-known thiamine antagonist and inhibitor of transketolase. Oxythiamine alters protein expression in a dose dependent manner. The level of alpha-enolase is increased by Oxythiamine treatment, while expression of 14-3-3 protein beta/alpha is suppressed by Oxythiamine at a stratified dose. Oxythiamine causes dynamic changes of total protein expression in time dependent fashion. Oxythiamine suppresses expression of cellular phosphor proteins significantly[2].
[1]. Moradi H, et al. Functional thiamine deficiency in end-stage renal disease: malnutrition despite ample nutrients. Kidney Int. 2016 Aug;90(2):252-254. [2]. Wang J, et al. Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells. Exp Hematol Oncol. 2013 Jul 27;2:18.
Cell experiment: | Human pancreatic carcinoma cell line MIA PaCa-2 is used throughout the study. Experiments are set up in two groups: dose and time-dependent groups. For the dose-dependent group, the cells are stimulated with 5, 50 and 500 μM Oxythiamine (OT) for 48 hours, respectively. The unstimulated cells are considered as control. For the time-dependent group, the cells are stimulated with 50 μM Oxythiamine in MEM containing natural amino acids or 50% of 15 N algal amino acid mixture for 12 and 48 h. The unstimulated cells are considered as the zero time point. Each treatment is repeated four times with 10 mL/flask. The cell pellets are then collected for further analysis[2]. |
References: [1]. Moradi H, et al. Functional thiamine deficiency in end-stage renal disease: malnutrition despite ample nutrients. Kidney Int. 2016 Aug;90(2):252-254. | |
| Cas No. | 136-16-3 | SDF | |
| 别名 | Hydroxythiamin | ||
| Canonical SMILES | CC1=C(CCO)SC=[N+]1CC2=CN=C(C)NC2=O | ||
| 分子式 | C12H16N3O2S | 分子量 | 266.34 |
| 溶解度 | DMSO : 51.67 mg/mL (194.00 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7546 mL | 18.773 mL | 37.546 mL |
| 5 mM | 0.7509 mL | 3.7546 mL | 7.5092 mL |
| 10 mM | 0.3755 mL | 1.8773 mL | 3.7546 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet