Oxytocin
(Synonyms: 缩宫素) 目录号 : GC16222
Oxytocin是一种多效的下丘脑肽，有助于分娩、哺乳和亲社会行为。
Cas No.:50-56-6
Sample solution is provided at 25 µL, 10mM.
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Oxytocin is a pleiotropic hypothalamic peptide that helps in childbirth, lactation and prosocial behavior[1]. Oxytocin can act as a stress response molecule with anti-inflammatory, antioxidant and neuroprotective effects[2]. Oxytocin can regulate the growth of cancer cells by activating oxytocin receptors[3].
In vitro, Oxytocin (1μM) treatment of SH-SY5Y cells for 12h significantly increased the amount of total protein in cell lysates and significantly reduced the concentration of neurotrophic growth factor (NGF)[4]. Treatment of human umbilical vein endothelial cells (HUVEC) with Oxytocin (1nM) stimulated cell migration and invasion[5]. Treatment of hTERTmyo and SK-UT-1 cells with Oxytocin (1μM) for 24-96h inhibited the proliferation of both cells in a time-dependent manner[6].
In vivo, Oxytocin (0.2mg/kg) was administered intranasally to treat valproic acid (VPA)-induced autistic mice, which improved the behavior of autistic mice, reduced anxiety, depression, and repetitive behaviors, and improved social interactions[7]. Oxytocin (0.1-0.3mg/kg) was administered subcutaneously to treat phencyclidine-induced hyperactive rats, which significantly reduced the hyperactive behavior of the rats and increased prosocial behavior[8].
References:
[1] Tarsha M S, Narvaez D. The evolved nest, oxytocin functioning, and prosocial development[J]. Frontiers in Psychology, 2023, 14: 1113944.
[2] Kamrani-Sharif R, Hayes A W, Gholami M, et al. Oxytocin as neuro-hormone and neuro-regulator exert neuroprotective properties: a mechanistic graphical review[J]. Neuropeptides, 2023, 101: 102352.
[3] Cassoni P, Sapino A, Marrocco T, et al. Oxytocin and oxytocin receptors in cancer cells and proliferation[J]. Journal of neuroendocrinology, 2004, 16(4): 362-364.
[4] Bakos J, Strbak V, Ratulovska N, et al. Effect of oxytocin on neuroblastoma cell viability and growth[J]. Cellular and molecular neurobiology, 2012, 32: 891-896.
[5] Cattaneo M G, Chini B, Vicentini L M. Oxytocin stimulates migration and invasion in human endothelial cells[J]. British journal of pharmacology, 2008, 153(4): 728-736.
[6] Busnelli M, Rimoldi V, Viganò P, et al. Oxytocin-induced cell growth proliferation in human myometrial cells and leiomyomas[J]. Fertility and sterility, 2010, 94(5): 1869-1874.
[7] Wang Y, Zhao S, Liu X, et al. Oxytocin improves animal behaviors and ameliorates oxidative stress and inflammation in autistic mice[J]. Biomedicine & Pharmacotherapy, 2018, 107: 262-269.
[8] Kohli S, King M V, Williams S, et al. Oxytocin attenuates phencyclidine hyperactivity and increases social interaction and nucleus accumben dopamine release in rats[J]. Neuropsychopharmacology, 2019, 44(2): 295-305.
Oxytocin是一种多效的下丘脑肽，有助于分娩、哺乳和亲社会行为[1]。Oxytocin能够作为一种压力应对分子，具有抗炎、抗氧化和神经保护作用[2]。Oxytocin能够通过激活催产素受体对癌细胞起生长调节作用[3]。
在体外，Oxytocin（1μM）处理SH-SY5Y细胞12h，显著增加了细胞裂解物中的总蛋白量，显著降低了神经营养生长因子（NGF）的浓度[4]。Oxytocin（1nM）处理人脐静脉内皮细胞（HUVEC），刺激了细胞迁移和侵袭[5]。Oxytocin（1μM）处理hTERTmyo和SK-UT-1细胞24-96h，以时间依赖性方式抑制了两种细胞的增殖[6]。
在体内，Oxytocin（0.2mg/kg）通过鼻腔给药治疗丙戊酸（VPA）诱导的自闭症小鼠，改善了自闭症小鼠的行为，减少了焦虑、抑郁和重复行为，并改善了社交互动[7]。Oxytocin（0.1-0.3mg/kg）通过皮下注射治疗苯环利定诱导的多动症大鼠，显著减少了大鼠多动行为，增加了亲社会行为[8]。
| Cell experiment [1]: | |
Cell lines | SH-SY5Y cells |
Preparation Method | Cells were incubated in presence of Oxytocin (1μM) for 12 or 96h. Brain-derived neurotrophic factor (BDNF) or Neurotrophic growth factor (NGF) was measured by ELISA. |
Reaction Conditions | 1μM; 12, 96h |
Applications | Neurotrophic Factors Oxytocin (1μM, 12h) significantly increased amount of total proteins measured in SH-SY5Y cell lysates. Concentration of BDNF related to proteins did not change significantly, while that of NGF significantly decreased in response to Oxytocin. No effect of Oxytocin on neurotrophic factors was observed after 24 or 48h. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | The pregnant mice were randomly divided into 2 groups: the control group and valproate (VPA) group. At E12.5, pregnant mice in the VPA group received VPA (600mg/kg; intraperitoneal injection) to induce autism of offsprings. Pregnant mice in the control group received equal amounts of normal saline. Male offspring were used for subsequent experiments. After identification of autism, the offspring were divided into 3 groups: the control group, autism group and autism+Oxytocin group. At postnatal day 30 (P30), offspring in autism+Oxytocin group received Oxytocin (0.2mg/kg; intranasal administration). 30min later, animal behavior tests were performed. |
Dosage form | 0.2mg/kg; intranasal administration |
Applications | Oxytocin improved the behaviors of autistic mice, with less anxiety, depression and repetitive behavior, and ameliorated social interaction. |
References: | |
| Cas No. | 50-56-6 | SDF | |
| 别名 | 缩宫素 | ||
| 分子式 | C43H66N12O12S2 | 分子量 | 1007.19 |
| 溶解度 | ≥ 182.8mg/mL in DMSO | 储存条件 | Store at 4°C, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9929 mL | 4.9643 mL | 9.9286 mL |
| 5 mM | 0.1986 mL | 0.9929 mL | 1.9857 mL |
| 10 mM | 0.0993 mL | 0.4964 mL | 0.9929 mL |
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动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度： mg/ml；
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2.
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- Purity: >98.00%
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