P7C3
目录号 : GC14692
A proneurogenic and neuroprotective compound
Cas No.:301353-96-8
Sample solution is provided at 25 µL, 10mM.
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Target: Nicotinamide phosphoribosyltransferase (NAMPT)
IC50: N/A
P7C3 is an orally bioavailable proneurogenic and neuroprotective chemical which targets NAMPT enzyme [1]. NAMPT, the rate-limiting enzyme in the salvage pathway plays a critical role in the conversion of nicotinamide into nicotinamide adenine mononucleotide (NMN) and nicotinamide adenine dinucleotide (NAD) [2].
In vitro: P7C3 (1, 10, and 100 nM) preserved mitochondrial membrane potential in parallel to proneurogenic activity [1]. Administration of P7C3 (5 μM) to U2OS cells treated with doxorubicin, which induced NAD depletion, induced an increasing in intracellular NAD levels and concomitant protection from doxorubicin-mediated toxicity through the NAMPT-mediated salvage [2].
In vivo: P7C3 (5 to 40 mg/kg, oral administration) showed its proneurogenic activity through protecting newborn neurons from apoptosis. In addition, P7C3 (10 mg/kg, oral administration) enhanced hippocampal neurogenesis, preserved cognitive capacity, and prevented weight loss in terminally aged rats [1]. P7C3 (437.5 μg, twice daily via i.p. injections) treatment restored hippocampal neurogenesis in the Ts65Dn mouse model of Down syndrome [3].
References:
1. Pieper AA, Xie S, Capota E, Estill SJ, Zhong J, Long JM, et al. Discovery of a proneurogenic, neuroprotective chemical. Cell. 2010;142(1):39-51.
2. Wang G, Han T, Nijhawan D, Theodoropoulos P, Naidoo J, Yadavalli S, et al. P7C3 neuroprotective chemicals function by activating the rate-limiting enzyme in NAD salvage. Cell. 2014;158(6):1324-34.
3. Latchney SE, Jaramillo TC, Rivera PD, Eisch AJ, Powell CM. Chronic P7C3 treatment restores hippocampal neurogenesis in the Ts65Dn mouse model of Down Syndrome [Corrected]. Neurosci Lett. 2015;591:86-92.
| Cas No. | 301353-96-8 | SDF | |
| 化学名 | 1-(3,6-dibromo-9H-carbazol-9-yl)-3-(phenylamino)propan-2-ol | ||
| Canonical SMILES | BrC1=CC(C2=C3C=CC(Br)=C2)=C(N3CC(O)CNC4=CC=CC=C4)C=C1 | ||
| 分子式 | C21H18Br2N2O | 分子量 | 474.19 |
| 溶解度 | ≥ 22.7mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1089 mL | 10.5443 mL | 21.0886 mL |
| 5 mM | 0.4218 mL | 2.1089 mL | 4.2177 mL |
| 10 mM | 0.2109 mL | 1.0544 mL | 2.1089 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet