PAMAM Dendrimer G3.0 Amidoethanol (water solution)

PAMAM dendrimer G3.0 amidoethanol (PAMAM G3.0 amidoethanol) is a polyamidoamine (PAMAM) dendrimer with hydroxyl termini.¹ It is approximately 30 Å in diameter in PBS and has 32 surface groups. PAMAM G3.0 amidoethanol is an antagonist for the pore-forming channels anthrax protective antigen 63 (PA63) in lipid membranes (IC₅₀ = 44.9 nM).² It inhibits aggregation of amylin containing a serine-to-glycine substitution at position 20 (amylin^S20G) in a concentration-dependent manner and amylin^S20G-induced toxicity in MIN6-K8 and NIT-1 pancreatic β-cells at 6 µM.³ Topical application of PAMAM G3.0 amidoethanol (1 mg/ml) encapsulating the synthetic glucocorticoid dexamethasone increases the levels of dexamethasone in the sclera, conjunctiva, and cornea in isolated rabbit eyes.⁴ It reduces tail growth in C. riparius larvae and induces DNA damage in a comet assay using C. riparius larval homogenates when used at concentrations of 10 or 100 ppm.⁵

References:
[1]. Dobrovolskaia, M.A., Patri, A.K., Simak, J., et al.Nanoparticle size and surface charge determine effects of PAMAM dendrimers on human platelets in vitroMol. Pharm.9(3)382-393(2012).
[2]. FÖrstner, P., Bayer, F., Kalu, N., et al.Cationic PAMAM dendrimers as pore-blocking binary toxin inhibitorsBiomacromolecules15(7)2461-2474(2014).
[3]. Gurzov, E.N., Wang, B., Pilkington, E.H., et al.Inhibition of hIAPP amyloid aggregation and pancreatic β-cell toxicity by OH-terminated PAMAM dendrimerSmall12(12)1615-1626(2016).
[4]. Yavuz, B., Pehlivan, S.B., Vural, ?., et al.In vitro/in vivo evaluation of dexamethasone--PAMAM dendrimer complexes for retinal drug deliveryJ. Pharm. Sci.104(11)3814-3823(2015).
[5]. PlanellÓ, R., Rosal, R., Aquilino, M., et al.Genotoxic effects and transcriptional deregulation of genetic biomarkers in Chironomus riparius larvae exposed to hydroxyl- and amine-terminated generation 3 (G3) polyamidoamine (PAMAM) dendrimersSci. Total Environ.774145828(2021).