PAR1 (1-6) (mouse, rat) (trifluoroacetate salt)
(Synonyms: H-Ser-Phe-Phe-Leu-Arg-Asn, PAR1-AP, Proteinase-Activated Receptor 1, SFFLRN, TRAP, Thrombin Receptor Activating Peptide) 目录号 : GC40159
A peptide agonist of PAR1
Sample solution is provided at 25 µL, 10mM.
PAR1 (1-6) is a hexapeptide that corresponds to amino acid residues 1-6 of the amino terminal tethered ligand sequence of mouse and rat proteinase-activated receptor 1 (PAR1), residues 42-47 of the full-length mouse sequence, and residues 46-51 of the full-length rat sequence. PAR1 is a high-affinity thrombin receptor that induces platelet activation and deposition into thrombi as well as smooth muscle mitogenesis. Unlike thrombin, PAR1 (1-6) acts as an agonist of PAR1 in smooth muscle cells but is inactive in rodent platelets. It increases mitogenesis and the expression of the IGF-1 receptor (IGF-1R) by 47% in vascular smooth muscle cells (VSMCs), an effect that is inhibited by the thrombin inhibitor hirudin and by the protein tyrosine kinase inhibitor genistein . PAR1 (1-6) increases histamine and β-hexosaminidase release from rat peritoneal mast cells in a concentration-dependent manner, with the maximum release at a concentration of 50 µM. It also mimics the effect of thrombin, increasing swelling-activated efflux of [3H]glutamate induced by hypoosmotic medium in astrocytes in vitro when used at concentrations ranging from 5 to 10 µM.
Cas No. | SDF | ||
别名 | H-Ser-Phe-Phe-Leu-Arg-Asn, PAR1-AP, Proteinase-Activated Receptor 1, SFFLRN, TRAP, Thrombin Receptor Activating Peptide | ||
分子式 | C37H54N10O9 • XCF3COOH | 分子量 | 782.9 |
溶解度 | Water: 1 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 1.2773 mL | 6.3865 mL | 12.773 mL |
5 mM | 0.2555 mL | 1.2773 mL | 2.5546 mL |
10 mM | 0.1277 mL | 0.6387 mL | 1.2773 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet