Parbendazole (SKF 29044)
(Synonyms: 帕苯咪唑,SKF 29044) 目录号 : GC32134An inhibitor of microtubule assembly
Cas No.:14255-87-9
Sample solution is provided at 25 µL, 10mM.
Parbendazole is an inhibitor of microtubule assembly (EC50 = 3 ?M).1 It reduces viability of HeLa cells (EC50 = 0.53 ?M) and induces genotoxic stress in a reporter assay using HEK293 cells.2 Parbendazole reduces, but does not eradicate, worm burden in goats with naturally acquired or experimental nematode infections when administered at a dose of 15 mg/kg.3 It is teratogenic to lamb embryos when administered to pregnant ewes at a dose of 60 mg/kg during the third week of pregnancy.
1.Havercroft, J.C., Quinlan, R.A., and Gull, K.Binding of parbendazole to tubulin and its influence on microtubules in tissue-culture cells as revealed by immunofluorescence microscopyJ. Cell Sci.49195-204(1981) 2.Lo, Y.-C., Senese, S., France, B., et al.Computational cell cycle profiling of cancer cells for prioritizing FDA-approved drugs with repurposing potentialSci. Rep.7(1)(2017) 3.Charles, T.P., Pompeu, J., and Miranda, D.B.Efficacy of three broad-spectrum anthelmintics against gastrointestinal nematode infections of goatsVet. Parasitol.34(1-2)71-75(1989)
Kinase experiment: | Pure tubulin is obtained from sheep brain by 2 cycles of assembly and disassembly in vitro. Immediately prior to use the protein is centrifuged at 130000 g for 30 min to remove any aggregates. It is used at a protein concentration of 0-2 mg/mL in 0.025 M Pipes buffer, 0-5 mM EGTA, 0-25 mM Mg2SOsup>4, 0.1 mM GTP. Drug binding is determined by equilibrium dialysis using concentrations of parbendazole between 0.1 μM and 4 μM, and 2% (v/v) DMF (dimethyl formamide) as a carrier. Equilibrium is achieved by constant stirring for 2 h at 26°C, bovine serum albumin being used as a standard. 200 μL aliquots are counted in PCS in a 25-200B liquid scintillation counter[2]. |
Cell experiment: | Vero cells, an established cell line derived from monkey kidney are seeded in DMEM supplemented with 10% (v/v) foetal calf serum onto glass coverslips in multiwell dishes. They are allowed to settle, and spread for 2-5 h in a humid atmosphere at 37°C. After this time the medium is changed to DMEM containing 2, 10 or 20 μM parbendazole and 1% (v/v) DMSO controls contained 1 % (v/v) DMSO or had no additions[2]. |
References: [1]. Lo YC, et al. Computational Cell Cycle Profiling of Cancer Cells for Prioritizing FDA-Approved Drugs with Repurposing Potential. Sci Rep. 2017 Sep 12;7(1):11261. |
Cas No. | 14255-87-9 | SDF | |
别名 | 帕苯咪唑,SKF 29044 | ||
Canonical SMILES | O=C(OC)NC1=NC2=CC=C(CCCC)C=C2N1 | ||
分子式 | C13H17N3O2 | 分子量 | 247.29 |
溶解度 | DMSO : 4 mg/mL (16.18 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 4.0438 mL | 20.2192 mL | 40.4384 mL |
5 mM | 0.8088 mL | 4.0438 mL | 8.0877 mL |
10 mM | 0.4044 mL | 2.0219 mL | 4.0438 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00%
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