PD-1 Protein, Mouse (HEK293, His-Fc)
目录号 : GC26686PD-1（程序性细胞死亡 1）蛋白负向调节免疫反应并影响细胞凋亡和耐受诱导等过程。
Sample solution is provided at 25 µL, 10mM.
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PD-1 (programmed cell death 1) protein negatively regulates immune responses and affects processes such as apoptosis and tolerance induction. It is a transmembrane protein found on the outside of the plasma membrane and expressed in the retina. PD-1 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived PD-1 protein, expressed by HEK293 , with C-hFc, C-8*His labeled tag.
| Purity | Greater than 95% as determined by reducing SDS-PAGE. | Source | HEK293 |
| Phycical Appearance | Lyophilized powder. | Shipping Condition | |
| Synonyms | Programmed cell death protein 1; PD1; CD279; PDCD1 | ||
| Amino Acid Sequence | LEVPNGPWRS LTFYPAWLTV SEGANATFTC SLSNWSEDLM LNWNRLSPSN QTEKQAAFCN GLSQPVQDAR FQIIQLPNRH DFHMNILDTR RNDSGIYLCG AISLHPKAKI EESPGAELVV TERILETSTR YPSPSPKPEG RFQ | ||
| Apparent Molecular Weight | 60-65 kDa | ||
| Stability | Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | 1. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625 ng/mL of bound hPD-L1. The ED50 for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×103 units/mg. 2. Immobilized Recombinant Mouse PD-1 at 1 μg/ml (100 μl/well) can bind Recombinant Mouse PD-L1. The ED50 for this effect is 0.5001 μg/mL. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625ng/mL of bound hPD-L1. The ED50 for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×103 units/mg. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4. | ||
PD-1 (programmed cell death 1) protein negatively regulates immune responses and affects processes such as apoptosis and tolerance induction. It is a transmembrane protein found on the outside of the plasma membrane and expressed in the retina. PD-1 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived PD-1 protein, expressed by HEK293 , with C-hFc, C-8*His labeled tag.
1. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625 ng/mL of bound hPD-L1. The ED50 for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×103 units/mg. 2. Immobilized Recombinant Mouse PD-1 at 1 μg/ml (100 μl/well) can bind Recombinant Mouse PD-L1. The ED50 for this effect is 0.5001 μg/mL. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625ng/mL of bound hPD-L1. The ED50 for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×103 units/mg.
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.