PDK1 inhibitor
(Synonyms: PDK1抑制剂,PDK1 inhibitor) 目录号 : GC12866
PDK1 inhibitor (PDK1 inhibitor) 是一种磷酸肌醇依赖性激酶-1 (PDK1) 抑制剂。
Cas No.:1001409-50-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | The human GBM cells (i.e., U87MG, U343MG, or ANGM-CSS) or the respective GSCs are seeded and incubated for the indicated times with the indicated concentrations of SA16 (1 nM to 100 μM), MP7 (2.5 nM, 25 nM, 250 nM and 2.5 μM), or Alisertib. When indicated, cells are treated with MP7 and Alisertib in combination. To verify GSC chemoresistance, U87MG or GSCs are incubated with 50 μM TMZ for 72 h. For the long-term treatment of cells, NSC or complete medium containing drugs is replaced every 3 days. Cell proliferation is determined using the MTS assay: the dehydrogenase activity in active mitochondria reduces MTS to the soluble formazan product, whose absorbance at 490 nm is measured with an automated plate reader. The mean background from each test condition is subtracted, and the data are expressed as the percentage of untreated cells (control). IC50 values are derived from the sigmoid dose-response curve. The percentage of inhibition is calculated as 100% minus the percentage of cell proliferation[1]. |
References: [1]. Daniele S, et al. Dual Inhibition of PDK1 and Aurora Kinase A: An Effective Strategy to Induce Differentiation and Apoptosis of Human Glioblastoma Multiforme Stem Cells. ACS Chem Neurosci. 2017 Jan 18;8(1):100-114. | |
PDK1 inhibitor is an potent and selective inhibitor of PDK1 with potential as anticancer agent. GSK 2334470,a inhibitor of PDK1, has IC50 value of 0.00251µM and CHEMBL1172241 with IC50 value of 0.085µM. [1]
PDPK1 stands for 3-phosphoinositide-dependent protein kinase 1, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK. [2]An important role for PDPK1 is in the signalling pathways activated by several growth factors and hormones including insulin signaling. PDPK1 functions downstream of PI3K through PDPK1's interaction with membrane phospholipids.[3]PI3K indirectly regulates PDPK1 by phosphorylating phosphatidylinositols which in turn generates phosphatidylinositol (3,4)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate. [4]However, PDPK1 is believed to be constitutively active and does not always require phosphatidylinositols for its activities. Phosphatidylinositols are only required for the activation at the membrane of some substrates including AKT. PDPK1 however does not require membrane lipid binding for the efficient phosphorylation of most of its substrates in the cytosol.PDK1 is implicated in the development and progression of melanomas.[5]Many cancer-driving mutations induce activation of PDK1 targets including Akt, S6K (p70 ribosomalS6 kinase) and SGK (serum- and glucocorticoid-induced protein kinase).
GSK2334470 is more effective at inhibiting PDK1 substrates that are activated in the cytosol rather than at the plasma membrane. Consistent with this, GSK2334470 inhibited Akt activation in knock-in embryonic stem cells expressing a mutant of PDK1 that is unable to interact with phosphoinositides more potently than in wild-type cells by Immunoblotting.[6]GSK2334470 also suppressed T-loop phosphorylation and activation of RSK2 (p90 ribosomal S6 kinase 2), another PDK1 target activated by the ERK(extracellular-signal-regulated kinase) pathway.[7] GSK2334470 will be useful in probing biological processes controlled by PDK1. Therefore, GSK2334470 is much more specific than other reported PDK1 inhibitors.
Reference:
1.MurphyST. et al. Discovery of novel, potent, and selective inhibitors of 3-phosphoinositide-dependent kinase (PDK1). J Med Chem. 2011, 54(24):8490-500.
2.Mora A, Komander D, van Aalten DM, Alessi DR. "PDK1, the master regulator of AGC kinase signal transduction". Semin. Cell Dev. Biol. 2004,15 (2): 161-70.
3.Vanhaesebroeck B, Alessi DR. "The PI3K-PDK1 connection: more than just a road to PKB". Biochem. J. 2000, 346 (3): 561-76.
4.Frödin M, Antal TL. Et al. "A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation". EMBO J. 2002, 21 (20): 5396-407.
5.Scortegagna, M.. et al. "Genetic inactivation or pharmacological inhibition of Pdk1 delays development and inhibits metastasis of BrafV600E::Pten-/- melanoma". Oncogene.2013.
6.Ayaz NAJAFOV. Characterization of GSK2334470, a novel and highly specific inhibitor of PDK1. Biochem. J. (2011) 433, 357-369 .
7.Tamguney, T. et al. Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells. Cell ,2008, 314, 2299–2312.
| Cas No. | 1001409-50-2 | SDF | |
| 别名 | PDK1抑制剂,PDK1 inhibitor | ||
| 化学名 | 1-[(3,4-difluorophenyl)methyl]-2-oxo-N-[(1R)-2-[(2-oxo-1,3-dihydrobenzimidazol-5-yl)oxy]-1-phenylethyl]pyridine-3-carboxamide | ||
| Canonical SMILES | C1=CC=C(C=C1)C(COC2=CC3=C(C=C2)NC(=O)N3)NC(=O)C4=CC=CN(C4=O)CC5=CC(=C(C=C5)F)F | ||
| 分子式 | C28H22F2N4O4 | 分子量 | 516.5 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9361 mL | 9.6805 mL | 19.3611 mL |
| 5 mM | 0.3872 mL | 1.9361 mL | 3.8722 mL |
| 10 mM | 0.1936 mL | 0.9681 mL | 1.9361 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。