PE 22-28 (acetate)
(Synonyms: Gly-Val-Ser-Trp-Gly-Leu-Arg-OH) 目录号 : GC91810PE 22-28 is a peptide inhibitor of the two-pore domain potassium channel K2P2.1/TREK1 (IC50 = 0.1 nM in HEK293 cells expressing the human channel) and a fragment of spadin .
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
PE 22-28 is a peptide inhibitor of the two-pore domain potassium channel K2P2.1/TREK1 (IC50 = 0.1 nM in HEK293 cells expressing the human channel) and a fragment of spadin .[1] It is selective for K2P2.1/TREK1 over K2P10.1 /TREK2, K2P13.1/TRAAK, K2P18.1/TRESK, and K2P3.1/TASK1 in HEK293 cells expressing the human channels at 100 nM. PE 22-28 (4 µg/kg for four days) increases neurogenesis and the levels of postsynaptic density protein 95 (Psd-95), a measure of increased synaptogenesis, in isolated mouse hippocampi. It decreases immobility in the forced swim test, latency to feed in the novelty-suppressed feeding test, and the time to escape in the Porsolt learned helplessness test in mice when administered at a dose of 0.3 µg/kg for four days.
References:
[1].Djillani, A., Pietri, M., Moreno, S., et al.Shortened spadin analogs display better TREK-1 inhibition, in vivo stability and antidepressant activityFront. Pharmacol.8:643(2017).
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.2922 mL | 6.4608 mL | 12.9216 mL |
| 5 mM | 0.2584 mL | 1.2922 mL | 2.5843 mL |
| 10 mM | 0.1292 mL | 0.6461 mL | 1.2922 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。