Penicillin G Sodium
(Synonyms: 青霉素G钠盐; 苄青霉素钠; Benzylpenicillin sodium salt) 目录号 : GC17181
青霉素G钠是一种典型的β-内酰胺类抗生素。
Cas No.:69-57-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Kinase experiment: | At 278 K, various concentrations of Penicillin G sodium salt, cefalexin and cefoxitin solutions are added to TEM-1β-lactamase solution (5×10-6 M). The concentrations of the three antibiotics are gradually increased from 0 to 25×10-6 M. Following mixing and interaction for 2 min, the Ultraviolet-visible (UV-Vis) absorption spectra are recorded on a spectrophotometer with a slit of 2 nm and scanning speed of 400 nm/min using 0.02 M phosphate buffer (pH 7.0) as a reference[1]. |
Animal experiment: | A randomized complete block design with 2 replicates is used for this study. Each replicate includes 448 pigs, with 16 pens and 28 pigs per pen. Pigs are also sorted by weight; such that animals of similar weight based on visual observation are grouped together within blocks. Two treatments are randomly assigned within each block of 2 contiguous pens using a formal randomization process. The treatment groups are Control (no treatment given) and Treated (Penicillin G sodium salt). Penicillin G sodium salt is administered via the drinking water for 5 d over 2 periods of treatment. The first treatment period commences on the day of weaning, when the pigs are moved into the nursery barns (Day 1) and ends on Day 5. The second treatment period begins on Day 21 and ends on Day 25. The Control group does not receive treatment[2]. |
References: [1]. Yang J, et al. Spectroscopic analysis and docking simulation on the recognition and binding of TEM-1 β-lactamase with β-lactam antibiotics. Exp Ther Med. 2017 Oct;14(4):3288-3298. | |
IC50: 0.002 µg/ml for Streptococcus pneumoniae serotype 4
Penicillin G sodium is a penicillin antibiotic against sensitive organisms during the stage of active multiplication and acts by inhibiting the biosynthesis of bacterial cell wall mucopeptides. It is not active against penicillinase-producing bacteria, which include many strains of staphylococci.
In vitro: Penicillin G sodium works by interfering with the formation of the bacteria's cell wall. This weakens the cell wall and kills the bacteria. Penicillin G is highly effective in-vitro against staphylococci, streptococci and pneumococci. Other organisms sensitive in-vitro to penicillin G are Neisseria gonorrhoeae, Bacillus anthracis, Corynebacterium diphtheriae, Clostridia et al. Treponema pallidum is extremely sensitive [1].
In vivo: With intermittent intramuscular administration of penicillin normal rats were cured after doses of 4 mg/kg/d, whereas decomplemented rats recovered only after daily doses of above 100 mg/kg. When penicillin was administered by way of continuous infusion, doses of 3.5 mg/kg/d were required for a cure of infections in both normal and decomplemented rats [2].
Clinical trials: Penicillin G sodium is used to treat a wide variety of bacterial infections. It is also used to prevent the heart infection in patients with certain heart diseases who are having surgery. Penicillin G sodium is known as a natural penicillin antibiotic. It works by stopping the growth of bacteria [1].
Reference:
[1] Bakker-Woudenberg IA, van den Berg JC, Fontijne P, Michel MF. Efficacy of continuous versus intermittent administration of penicillin G in Streptococcus pneumoniae pneumonia in normal and immunodeficient rats. Eur J Clin Microbiol. 1984 Apr;3(2):131-5.
| Cas No. | 69-57-8 | SDF | |
| 别名 | 青霉素G钠盐; 苄青霉素钠; Benzylpenicillin sodium salt | ||
| 化学名 | sodium;(2S,5R,6R)-3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate | ||
| Canonical SMILES | CC1(C(N2C(S1)C(C2=O)NC(=O)CC3=CC=CC=C3)C(=O)[O-])C.[Na+] | ||
| 分子式 | C16H18N2O4S.Na | 分子量 | 356.37 |
| 溶解度 | ≥ 13.7mg/mL in DMSO | 储存条件 | Store at -20°C,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.8061 mL | 14.0304 mL | 28.0607 mL |
| 5 mM | 0.5612 mL | 2.8061 mL | 5.6121 mL |
| 10 mM | 0.2806 mL | 1.403 mL | 2.8061 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。