Pertussis Toxin
(Synonyms: 百日咳毒素) 目录号 : GC17532百日咳毒素是一种基于蛋白质的 AB5 型外毒素,由百日咳博德特氏菌产生,可引起百日咳。
Cas No.:70323-44-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >97.00%
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- SDS (Safety Data Sheet)
- Datasheet
Pertussis toxin (islet-activating protein) is a toxin, first isolated from B. pertussis, that is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the α subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/o proteins.[1],[2] Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals and induce EAE, an animal model of multiple sclerosis.[3],[4] Pertussis toxin was first described as an islet-activating protein because it caused a sustained potentiation of the secretory response of pancreatic islet cells to various stimuli that stimulate Gi-linked α-adrenergic receptors.[5],[6]
百日咳毒素(胰岛激活蛋白)是一种毒素,最初从百日咳杆菌中分离出来,用于研究细胞中的G蛋白偶联受体信号传导和动物实验性自身免疫性脑脊髓炎(EAE)。 百日咳毒素促进将NAD的ADP核苷酸转移给异源三聚体Gi / o蛋白的α亚基,导致受体与Gi / o蛋白解耦。 百日咳毒素也被用作佐剂,与特定抗原一起给动物接种以诱发多发性硬化症的动物模型EAE。 百日咳毒素最初被描述为胰岛激活蛋白,因为它引起了胰岛细胞对各种刺激产生持久增强的分泌反应,并刺激了Gi-连接α肾上腺能受体。
Reference:
[1]. Kaslow, H.R., and Burns, D.L. Pertussis toxin and target eukaryotic cells: Binding, entry, and activation. FASEB J. 6(9), 2684-2690 (1992).
[2]. Ui, M. Islet-activating protein, pertussis toxin: A probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase. Trends Pharmacol. Sci. 5, 277-279 (1984).
[3]. Hofstetter, H.H., Shive, C.L., and Forsthuber, T.G. Pertussis toxin modulates the immune response to neuroantigens injected in incomplete Freund’s adjuvant: Induction of Th1 cells and experimental autoimmune encephalomyelitis in the presence of high frequencies of Th2 cells. Journal of Immunology 169(1), 117-125 (2002).
[4]. Ronchi, F., Basso, C., Preite, S., et al. Experimental priming of encephalitogenic Th1/Th17 cells requires pertussis toxin-driven IL-1β production by myeloid cells. Nat.Commun. 7:11541, (2016).
[5]. Heyworth, C.M., Grey, A.M., Wilson, S.R., et al. The action of islet activating protein (pertussis toxin) on insulin’s ability to inhibit adenylate cyclase and activate cyclic AMP phosphodiesterases in hepatocytes. Biochemistry Journal 235(1), 145-149 (1986).
[6]. Katada, T., and Michio, U. Slow interaction of islet-activating protein with pancreatic islets during primary culture to cause reversal of α-adrenergic inhibition of insulin secretion. The Journal of Biological Chemisty 255(20), 9580-9588 (1980).
Cas No. | 70323-44-3 | SDF | |
别名 | 百日咳毒素 | ||
分子式 | C87H162N14O16S2 | 分子量 | 1724.44 |
溶解度 | Water: soluble | 储存条件 | Store at 2-8°C, do not freeze |
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.5799 mL | 2.8995 mL | 5.799 mL |
5 mM | 0.116 mL | 0.5799 mL | 1.1598 mL |
10 mM | 0.058 mL | 0.2899 mL | 0.5799 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。