Phenytoin-d10
(Synonyms: 苯妥英D10,5,5-Diphenylhydantoin-d10) 目录号 : GC48861
An internal standard for the quantification of phenytoin
Cas No.:65854-97-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Phenytoin-d10 is intended for use as an internal standard for the quantification of phenytoin by GC- or LC-MS. Phenytoin is an anticonvulsant agent and active metabolite of fosphenytoin . Phenytoin is formed from fosphenytoin by tissue phosphatases.1 It inhibits neuronal voltage-gated sodium channels in a voltage-dependent manner.2 Phenytoin reduces the neuronal firing frequency and decreases the amplitude of excitatory post-synaptic potentials (EPSPs) in electrically stimulated rat corticostriatal slices (EC50s = 42.8 and 33.5 µM, respectively).3 It protects against seizures induced by maximal electroshock (MES) in mice (ED50 = 10 mg/kg).4 Formulations containing phenytoin have been used in the treatment of tonic-clonic seizures and status epilepticus.
1.Boucher, B.A.Fosphenytoin: A novel phenytoin prodrugPharmacotherapy16(5)777-791(1996) 2.Yaari, Y., Selzer, M.E., and Pincus, J.H.Phenytoin: Mechanisms of its anticonvulsant actionAnn. Neurol.20(2)171-184(1986) 3.Calabresi, P., Centonze, D., Marfia, G.A., et al.An in vitro electrophysiological study on the effects of phenytoin, lamotrigine and gabapentin on striatal neuronsBr. J. Pharmacol.126(3)689-696(1999) 4.Brouillette, W.J., Jestkov, V.P., Akhtar, M.S., et al.Bicyclic hydantoins with a bridgehead nitrogen. Comparison of anticonvulsant activities with binding to the neuronal voltage-dependent sodium channelJ. Med. Chem.37(20)3289-3293(1994)
| Cas No. | 65854-97-9 | SDF | |
| 别名 | 苯妥英D10,5,5-Diphenylhydantoin-d10 | ||
| Canonical SMILES | O=C1C(C2=C([2H])C([2H])=C([2H])C([2H])=C2[2H])(C3=C([2H])C([2H])=C([2H])C([2H])=C3[2H])NC(N1)=O | ||
| 分子式 | C15H2D10N2O2 | 分子量 | 262.3 |
| 溶解度 | DMSO : 50 mg/mL (190.60 mM; Need ultrasonic) | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8124 mL | 19.0621 mL | 38.1243 mL |
| 5 mM | 0.7625 mL | 3.8124 mL | 7.6249 mL |
| 10 mM | 0.3812 mL | 1.9062 mL | 3.8124 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A simple isotope dilution electrospray ionization tandem mass spectrometry method for the determination of free phenytoin
Ther Drug Monit 2013 Dec;35(6):831-5.PMID:23942538DOI:10.1097/FTD.0b013e31828f5337
Background: Phenytoin (diphenylhydantoin) is an anticonvulsant drug frequently prescribed for the treatment of many types of seizures. Because the drug is highly protein bound (90%-95%) and many conditions can displace the drug from proteins, the measurement of free phenytoin is warranted. Due to the unavailability of free phenytoin assays in many chemistry analyzers or limitations of immunoassays, chromatographic methods such as liquid chromatography-tandem mass spectrometry (LC-MS-MS) are preferred for the assay of free phenytoin. Methods: The sample preparation involved ultrafiltration of serum or plasma to separate free phenytoin. Acetonitrile containing internal standard, Phenytoin-d10, was added to the ultrafiltrate. The samples were centrifuged, and supernatants were injected into an LC-MS-MS involving reverse phase Ultra BiPh 5-μm × 50 × 2.1-mm analytical column, and mobile phases, water and methanol containing 0.1% formic acid. The mass/charge (m/z) transitions were as follows: phenytoin -253.0 > 182.2 and 253.0 > 104.00; Phenytoin-d10 -263.2 > 192.12. Results: Linearity of the method ranged from 0.1 to 4.0 μg/mL. Within-run and between-run imprecision values were <5% and <10%, respectively. The samples were stable for 2 weeks at 4°C and 4 weeks at -20°C. The method compared well with the laborious liquid-liquid extraction method and did not show any significant ion suppression or enhancement. Conclusions: A simple LC-MS-MS method was developed for the assay of free phenytoin. The method does not require laborious liquid-liquid or solid-phase extraction. The method has high analytical sensitivity, low imprecision, and a wide analytical measurement range.