Phloxine B
(Synonyms: 酸性红92) 目录号 : GC44630
A red dye
Cas No.:18472-87-2
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Phloxine B is a red dye, the USP grade of which is used as a color additive for food, drugs, and cosmetics. It has also been used as an alternative to Gram staining, to differentiate between Gram-negative and Gram-positive bacteria. At 100 µg/ml, it can inhibit methicillin-resistant S. aureus growth in vitro. Phloxine B has also been shown to modulate channel activity of wild-type cystic fibrosis transmembrane conductance regulator chloride channels (CFTR; Ki = 38 µM) and mutant delta F508 CFTR (Ki = 33 µM).
| Cas No. | 18472-87-2 | SDF | |
| 别名 | 酸性红92 | ||
| Canonical SMILES | BrC1=C([O-])C(Br)=CC2=C1OC3=C(Br)C([O-])=C(Br)C=C3C24C(C(Cl)=C(Cl)C(Cl)=C5Cl)=C5C(O4)=O.[Na+].[Na+] | ||
| 分子式 | C20H2Br4Cl4O5•2Na | 分子量 | 829.6 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 2 mg/ml,PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.2054 mL | 6.027 mL | 12.054 mL |
| 5 mM | 0.2411 mL | 1.2054 mL | 2.4108 mL |
| 10 mM | 0.1205 mL | 0.6027 mL | 1.2054 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Low concentration Phloxine B staining for high chemical contrast, nonlinear microscope mosaic imaging of skin alterations in pseudoxanthoma elasticum
Biomed Opt Express 2021 Dec 8;13(1):252-261.PMID:35154868DOI:10.1364/BOE.443507.
Pseudoxanthoma elasticum (PXE) is an autosomal recessive metabolic disorder characterized by ectopic mineralization of soft connective tissue. Histopathology findings include fragmented, mineralized elastic fibers and calcium deposits in the mid-dermis. Nonlinear microscopy (NLM) can be used for visualization of these histopathological alterations of the mid-dermis in PXE-affected skin sections. Upon introducing a normalized 3D color vector representation of emission spectra of three of the main tissue components (collagen, elastin and calcification) we found that due to their broad, overlapping emission spectra, spectral separation of emission from elastin and calcification is practically impossible in fresh-frozen or unstained, deparaffinized PXE sections. However, we found that the application of a low concentration Phloxine B staining after the deparaffinization process creates an imaging contrast for these two tissue components, which enables spectral decomposition of their fluorescence images. The obtained concentration maps for calcium deposits can be well suited for the determination of illness severity by quantitative analysis.
Phloxine B interacts with the cystic fibrosis transmembrane conductance regulator at multiple sites to modulate channel activity
J Biol Chem 2002 May 31;277(22):19546-53.PMID:11904291DOI:10.1074/jbc.M108023200.
The fluorescein derivative Phloxine B is a potent modulator of the cystic fibrosis transmembrane conductance regulator (CFTR). Low micromolar concentrations of Phloxine B stimulate CFTR Cl(-) currents, whereas higher concentrations of the drug inhibit CFTR. In this study, we investigated the mechanism of action of Phloxine B. Phloxine B (1 microm) stimulated wild-type CFTR and the most common cystic fibrosis mutation, DeltaF508, by increasing the open probability of phosphorylated CFTR Cl(-) channels. At each concentration of ATP tested, the drug slowed the rate of channel closure without altering the opening rate. Based on the effects of fluorescein derivatives on transport ATPases, these data suggest that Phloxine B might stimulate CFTR by binding to the ATP-binding site of the second nucleotide-binding domain (NBD2) to slow the dissociation of ATP from NBD1. Channel block by Phloxine B (40 microm) was voltage-dependent, enhanced when external Cl(-) concentration was reduced and unaffected by ATP (5 mm), suggesting that Phloxine B inhibits CFTR by occluding the pore. We conclude that Phloxine B interacts directly with CFTR at multiple sites to modulate channel activity. It or related agents might be of value in the development of new treatments for diseases caused by the malfunction of CFTR.
Phloxine B, a versatile bacterial stain
FEMS Immunol Med Microbiol 2007 Mar;49(2):261-5.PMID:17328759DOI:10.1111/j.1574-695X.2007.00188.x.
The data presented suggest that Phloxine B, a color additive for food, drugs, and cosmetics has a potential use as a nontoxic, faster (<2 min), inexpensive (350 tests for <1 cent material) and simpler to use alternative to Gram staining. Using Phloxine B staining it was possible to differentiate among gram-negative and gram-positive bacteria by visual determination under normal room lighting, light microscopy, fluorescence microscopy and confocal microscopy. This work demonstrated that Phloxine B can be used as a differential versatile bacterial stain and establishes a correlation between the staining properties of the dye and its bactericidal effect.
Photolysis of Phloxine B in water and aqueous solutions
Arch Environ Contam Toxicol 1998 Oct;35(3):397-403.PMID:9732469DOI:10.1007/s002449900394.
Phloxine B (2',4',5',7'-tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt) as a potential photoactive insecticide was rapidly photodegraded in water under various light sources. Two major photolytic products characterized were 2',4',5'-tribromo-4,5,6, 7-tetrachlorofluorescein and 4',5'-dibromo-4,5,6, 7-tetrachlorofluorescein. The photolysis rates of Phloxine B were influenced by various factors including salts in medium, sample pH, and light sources. Half-lives (t(1/2)) of Phloxine B spiked in different water samples and 2% NaCl solution at 29 +/- 1 degreesC ranged from 0.70 to 1.28, 26.3 to 115, and 14.1 to 46.2 hours under 254 nm, 365 nm, and cool white fluorescent lights, respectively. Half-lives of Phloxine B in tap, stream, or seawater in a beaker were from 10 to 13 min under sunlight at ambient air temperature. In a range of buffer pH 6-8 at 29 +/- 1 degreesC, Phloxine B photodegraded slightly faster in acidic solution than in basic solution. The photolysis t(1/2) of Phloxine B at 29 +/- 1 degreesC was 25, 32, 128, and 755 min in the buffered NaF, NaCl, NaBr, and NaI solutions, respectively. The t(1/2) of Phloxine B was 31 min when Phloxine B was dissolved in the sodium phosphate buffer as control. Sodium iodide and ammonium iodide photostabilized Phloxine B 24 and 27 folds, respectively, when it was compared with the buffer control.
Phloxine B as a probe for entrapment in microcrystalline cellulose
Molecules 2012 Feb 7;17(2):1602-16.PMID:22314381DOI:10.3390/molecules17021602.
The photophysical behaviour of Phloxine B adsorbed onto microcrystalline cellulose was evaluated by reflectance spectroscopy and laser induced time-resolved luminescence in the picosecond-nanosecond and microsecond-millisecond ranges. Analysis of the absorption spectral changes with concentration points to a small tendency of the dye to aggregate in the range of concentrations under study. Prompt fluorescence, phosphorescence and delayed fluorescence spectral decays were measured at room temperature and 77 K, without the need of sample degassing because cellulose protects triplet states from oxygen quenching. In all cases, spectral changes with time and lifetime distribution analysis were consistent with the dye coexisting in two different environments: dyes tightly entrapped between polymer chains in crystalline regions of cellulose showed longer fluorescence and phosphorescence lifetimes and more energetic triplet states, while dyes adsorbed in more amorphous regions of the support showed shorter lifetimes and less energetic triplet states. This behaviour is discussed in terms of the different dye-support interactions in both kinds of adsorption sites.