Phosalone
(Synonyms: 伏杀磷) 目录号 : GC32226
Phosalone为非内吸性的有机磷杀虫、杀螨剂。主要用来防治棉花、小麦、烟叶、蔬菜、果树等作物抗性蚜螨和水稻蓟马、叶蝉、飞虱、螟虫、小麦黏虫、烟青虫等。
Cas No.:2310-17-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Phosalone is a member of the organophosphate family of insecticides. It is used as both an insecticide and acaricide.
| Cas No. | 2310-17-0 | SDF | |
| 别名 | 伏杀磷 | ||
| Canonical SMILES | S=P(OCC)(SCN1C(OC2=CC(Cl)=CC=C12)=O)OCC | ||
| 分子式 | C12H15ClNO4PS2 | 分子量 | 367.81 |
| 溶解度 | DMSO : ≥ 41 mg/mL (111.47 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7188 mL | 13.594 mL | 27.188 mL |
| 5 mM | 0.5438 mL | 2.7188 mL | 5.4376 mL |
| 10 mM | 0.2719 mL | 1.3594 mL | 2.7188 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Degradation of Phosalone by silver ion catalytic hydrolysis
Toxicol Mech Methods 2021 Mar;31(3):182-187.PMID:33287621DOI:10.1080/15376516.2020.1861672.
Pesticides application is expanding globally as the worldwide population increases demanding a secure and safe food supply. Organophosphorus (OP) pesticides, as a group, are widely used because they are rapidly degraded in the environment and because they have excellent efficacy and an acceptable price point. However, the chemical fate of organophosphorus pesticides is influenced by several factors, including their chemistry in aquatic environments. Among many degradation choices, hydrolysis by metal ions appears to be a good approach. Dissolved metal ions have been shown to promote the hydrolysis of organophosphorus pesticides. Using silver ion, we showed the effectiveness under in vitro and in vivo conditions for this metal ion to decontaminate water polluted by the organophosphorus Phosalone. Phosalone was completely degraded in the presence of silver ions in a mole ratio of 7:1 in 20 min. Rainbow trout were divided into experimental groups to investigate the most effective ratio of silver/Phosalone for pesticide degradation. Silver ion (2%) at a concentration of 0.75 and 0.1 mL removed Phosalone (2%) at concentrations of 0.4 and 0.5 mL. All the rainbow trout survived in these two groups. This experiment suggested that silver ions can be beneficial at ratios in the range of 1:4 to 1:6 by hydrolyzing Phosalone by attacking the electron-deficient phosphorus atom in the pesticide.
Phosalone-induced inflammation and oxidative stress in the colon: Evaluation and treatment
World J Gastroenterol 2016 Jun 7;22(21):4999-5011.PMID:27275092DOI:10.3748/wjg.v22.i21.4999.
Aim: To investigate the side effects of Phosalone on intestinal cells and to evaluate benefits of ellagic acid (EA) as a remedy. Methods: In order to conduct an in vivo study, a rat model was used. The rats were divided into ten groups based on the materials used in the experiment and their dosage. The first group was fed normally. The second group was administered EA through gavage. Next Four groups were given (1/3, 1/5, 1/10, 1/20) LD50 Phosalone; an organophosphorus compound. The last four groups received (1/3, 1/5, 1/10, 1/20) LD50 Phosalone and of EA. After one month, the rats were sacrificed and their colon cells were examined to evaluate the level of inflammation, proteins and oxidative stress markers. Results: The results of this research show that Phosalone elevates oxidative stress and changes the level of tumor necrosis factor-a (TNF-伪), interlukin-6尾 (IL-6尾) and nuclear factor (NF)-魏B proteins. EA administration reduced Phosalone toxicity and changed oxidative stress and inflammatory markers for all Phosalone doses. Overall changes in reduction of TNF-伪 (230.47 卤 16.55 pg/mg protein vs 546.43 卤 45.24 pg/mg protein, P < 0.001), IL-6尾 (15.85 卤 1.03 pg/mg protein vs 21.55 卤 1.3 pg/mg protein, P < 0.05), and NF-魏B (32.47 卤 4.85 pg/mg protein vs 51.41 卤 0.71 pg/mg protein, P < 0.05) manifest that the efficacy of EA is more viable for 1/3 LD50 dose of Phosalone. Furthermore, EA is effective to counteract the negative outcomes of oxidative stress. When EA was used to treat 1/3 LD50 of Phosalone's side effects, it improved the level of AChE activity (48.5% 卤 6% vs 25% 卤 7%, P < 0.05), TTM (0.391 卤 0.008 mmol/L vs 0.249 卤 0.032 mmol/L, P < 0.05), FRAP (46.04 卤 5.005 渭mol/L vs 18.22 卤 1.9 渭mol/L, P < 0.01) and MPO (0.222 卤 0.019 U/mg protein vs 0.387 卤 0.04 U/mg protein, P < 0.05). Conclusion: This research highlights that EA is effective to alleviate the side effects of Phosalone by reducing the level of oxidative stress and inflammatory proteins.
Side effects of chlorantraniliprole, Phosalone and spinosad on the egg parasitoid, Trichogramma brassicae
Ecotoxicology 2020 Sep;29(7):1052-1061.PMID:32448953DOI:10.1007/s10646-020-02235-y.
The combined use of chemicals and biological control is not always a successful strategy owing to the potential side effects on biocontrol agents. Lethal and sublethal effects of three commonly used insecticides were assessed on adult and immature stages of the egg parasitoid Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae). Recommended field concentrations of chlorantraniliprole, Phosalone and spinosad caused mortality on preimaginal stages by 24, 87, and 98%, respectively. Lethal effects on parasitoid adults exposed to the insecticide dry residues were estimated as median lethal concentrations (LC50) that were 13.28, 0.25, and 0.03 碌g a.i. ml-1 for chlorantraniliprole, Phosalone, and spinosad, respectively. The effect of a low lethal concentration (LC30) of the compounds was evaluated on various adult biological traits, such as longevity, fecundity, emergence rate and other life table parameters. All compounds caused detrimental effects on all the estimated demographical indexes. Chlorantraniliprole affected the net reproductive rate, mean generation time and doubling time in comparison to the control; while, Phosalone and spinosad adversely affected all assessed parameters. Phosalone and spinosad significantly reduced gross reproductive rate, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time and doubling time and reduced longevity, fecundity, emergence rate related to other biological parameters in comparison with control. The results suggest that all compounds are not fully compatible with the activity of T. brassicae, and that the inclusion of chlorantraniprole, spinosad and Phosalone into Integrated Pest Management (IPM) involving this parasitoid has to be avoided. Nevertheless, further studies in open field conditions and on a multiple generation scale are necessary for providing a more definitive conclusion on the IPM suitability of the three tested insectcides.
Effects of Phosalone plant pesticide on sperm parameters and sexual hormone levels in Wistar rats: An experimental study
Int J Reprod Biomed 2020 Sep 20;18(9):785-794.PMID:33062924DOI:10.18502/ijrm.v13i9.7683.
Background: Phosalone is an organophosphate insecticide, applied to control of plant pests. This compound has various side effects because it acts as an acetyl cholinesterase enzyme inhibitor. Objective: To investigate the effects of Phosalone on the sperm parameters of and levels of sex hormones in adult male rats. Materials and methods: In this experimental study, 16 adult (8-12 wk) male Wister rates (weighing 220-280 gr) were randomly assigned into 4 groups (n = 4/each). Group 1 (control) received only routine adequate water and food; Group 2, 3, and 4 received different low doses of Phosalone (60, 90, and 120 mg/kg respectively). The rats were weighed and anesthetized after 48 days. Sperm parameters including number, motility, and viability as well as sex hormones (such as Luteinizing Hormone, Follicle Stimulating Hormone, and testosterone) were evaluated and compared after removing the epididymis tail. Results: Our results showed that Phosalone decreased sperm motility, viability, and number in a dose-dependent manner. The level of FSH and LH was increased, and testosterone was decreased. Also, depending on the dose, Phosalone decrease sperm motility and viability (p 鈮?0.001), while the level of FSH and LH was increased and testosterone was decreased (p = 0.861). Conclusion: Phosalone has negative effects on reproductive indices in male rats and can cause serious damage and decrease the number and sperms motility. It can also cause infertility due to changing the concentration of hormones.
Nitrogen-doped carbon dots for dual-wavelength excitation fluorimetric assay for ratiometric determination of Phosalone
Mikrochim Acta 2021 Jul 9;188(8):247.PMID:34244909DOI:10.1007/s00604-021-04900-3.
N-doped carbon dots (N-CDs) were fabricated in a simple procedure by hydrothermal treatment of cellobiose and urea. When excited at 235 nm or 327 nm, only one emission peak at around 420 nm has been observed. With the addition of Phosalone, the excitation band at 235 nm was efficiently quenched within 1 min, while the excitation band at 327 nm showed little change. Accordingly, the fluorescence of the N-CDs-phosalone mixture showed quenching under 254-nm UV light, while nearly no fluorescence quenching could be observed under 365-nm UV light. This phenomenon provides a novel anti-false-positive mechanism for Phosalone identification. Therefore, the label-free ratiometric sensor for rapid, naked-eye, and anti-false-positive detection of Phosalone was proposed for the first time based on the intrinsic dual-excitation N-CDs. Under the optimum experimental conditions, the linear ranges of the excitation-based ratiometric assay were 0.08~4.0 渭g/mL and 4.0~14.0 渭g/mL; the limit of detection was 28.5 ng/mL. The as-constructed sensor was applied to detect Phosalone residue in actual samples, and results were compared with the standard gas chromatographic (GC) method. The recoveries of the established sensor were between 90.0% and 110.0% with RSD lower than 6.6%, while that for the GC method was between 92.5% and 113.0% with RSD lower than 5.8%. Results reveal that the accuracy (recovery) and precision (RSD) of the as-constructed method are comparable to the standard GC method. In this paper, dual-excitation N-doped carbon dots (N-CDs) were synthesized by a simply one-step hydrothermal method for the first time. The novel dual-excitation ratiometric sensor based on the sole intrinsic N-CDs was constructed for Phosalone sensing.