Phosmet
(Synonyms: 亚胺硫磷) 目录号 : GC47957
An organophosphate insecticide
Cas No.:732-11-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Phosmet is an organophosphate insecticide and acaricide.1,2,3 It reduces apple damage by a large variety of insects, including apple maggots, codling moths, and obliquebanded leafrollers when used as either a border or cover spray at a concentration of 1.9 kg AI/hectare.2 Phosmet is effective in controlling S. scabiei in pigs when applied as a 20% pour-on solution.3 It is toxic to rats via oral administration (LC50 = 230 mg/kg).4 Formulations containing phosmet have been used in the control of insects and mites in agriculture.
1.Abdollahi, M., and Karami-Mohajeri, S.A comprehensive review on experimental and clinical findings in intermediate syndrome caused by organophosphate poisoningToxicol. Appl. Pharmacol.258(3)309-314(2012) 2.Trimble, R.M., and Vickers, P.M.Evaluation of border sprays for managing the codling moth (Tortricidae: Lepidoptera) and the apple maggot (Tephritidae: Diptera) in Ontario apple orchardsJ. Econ. Entomol.93(3)777-787(2000) 3.Hewett, G.R.Phosmet for the systemic control of pig mange in growing pigsVet. Parasitol.18(3)265-268(1985) 4.Authority, E.F.S.Conclusion on the peer review of the pesticide risk assessment of the active substance phosmetEFSA J.9(5)2162(2011)
| Cas No. | 732-11-6 | SDF | |
| 别名 | 亚胺硫磷 | ||
| Canonical SMILES | O=C1N(CSP(OC)(OC)=S)C(C2=CC=CC=C21)=O | ||
| 分子式 | C11H12NO4PS2 | 分子量 | 317.3 |
| 溶解度 | Chloroform: Slightly Soluble,Methanol: Slightly Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1516 mL | 15.758 mL | 31.5159 mL |
| 5 mM | 0.6303 mL | 3.1516 mL | 6.3032 mL |
| 10 mM | 0.3152 mL | 1.5758 mL | 3.1516 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Peer review of the pesticide risk assessment of the active substance Phosmet
EFSA J 2021 Mar 17;19(3):e06237.PMID:33747228DOI:10.2903/j.efsa.2021.6237.
The conclusions of the EFSA following the peer review of the initial risk assessments carried out by the competent authorities of the rapporteur Member State, Spain, and co-rapporteur Member State, Greece, for the pesticide active substance Phosmet and the assessment of applications for maximum residue levels (MRLs) are reported. The context of the peer review was that required by Commission Implementing Regulation (EU) No 844/2012, as amended by Commission Implementing Regulation (EU) No 2018/1659. The conclusions were reached on the basis of the evaluation of the representative uses of Phosmet as an insecticide on citrus fruits, pome fruits, peaches/nectarines and potatoes (field uses). The reliable end points, appropriate for use in regulatory risk assessment, are presented. Missing information identified as being required by the regulatory framework is listed. Concerns are identified.
Phosmet: O,O-dimethyl S-phthalimidomethyl phospho-rodithio-ate
Acta Crystallogr Sect E Struct Rep Online 2010 Jul 31;66(Pt 8):o2137.PMID:21588425DOI:10.1107/S1600536810029338.
In the title compound, C(11)H(12)NO(4)PS(2), the dihedral angle between the phthalimidyl ring plane and the PS(2) plane of the phospho-rodithio-ate group is 60.41 (3)°. In the crystal structure, weak inter-molecular C-H⋯O hydrogen bonds and S⋯S inter-actions [3.3825 (9) Å] contribute to the stabilization of the packing.
Multispectroscopic studies of binding interaction of Phosmet with bovine hemoglobin
Spectrochim Acta A Mol Biomol Spectrosc 2023 Aug 5;296:122630.PMID:37001261DOI:10.1016/j.saa.2023.122630.
Phosmet is a phthalimide derived broad spectrum organophosphate pesticide which is vastly used across the globe to protect several ornamental or horticulture crops. The toxicity of Phosmet is of utmost concern because of its direct effect on the nervous system of the victim after exposure. The mechanism of Phosmet toxicity was explored by the interaction with the model blood protein which is hemoglobin. Bovine Hemoglobin (BHb) is a major protein of red blood cells (RBCs) that plays an important role in the exchange of gases for respiration and ensures adequate oxygen supply to tissues for oxygenation. In the current study, the interaction of BHb with Phosmet was revealed using various spectroscopic techniques. Circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) studies of BHb in the presence of Phosmet showed secondary structural changes in the protein post binding, Fluorescence study shows the involvement of the dynamic quenching predominantly, Van't Hoffs thermodynamic study showed negative enthalpy value and free energy change and negative entropy change that revealed the involvement of hydrogen bonding and van der Waal forces predominantly further revealing spontaneous nature of binding interaction. The shift in Ultraviolet-visible spectra also revealed the nature of the interaction. In-silico study finally deduced the involvement of hydrogen bonding and polar interaction. The study inferred the moderate interaction of BHb with Phosmet.
Spectroscopic and molecular modelling study of binding mechanism of bovine serum albumin with Phosmet
Spectrochim Acta A Mol Biomol Spectrosc 2021 Jan 5;244:118803.PMID:32829155DOI:10.1016/j.saa.2020.118803.
Phosmet exerts its neurotoxicity by inhibiting acetylcholinesterase that catalyzes the degradation of acetylcholine (a neurotransmitter). Serum proteins are known to influence the biodistribution of various endogenous and exogenous compounds. In the present study, the binding interactions of Phosmet with bovine serum albumin (BSA) was investigated to determine the free concentration of Phosmet for its neurotoxicity. The binding mechanism was studied using fluorescence, UV-Vis absorption spectroscopy, circular dichroism (CD), and molecular docking techniques. UV-Vis absorption data showed an increase in absorbance of BSA upon binding with Phosmet with a slight red-shift in the peak around 280 nm. Intrinsic fluorescence of BSA was quenched in the presence of Phosmet. The quenching was observed to be inversely correlated to the temperature that indicated the formation of ground state non-fluorescent complex (static quenching). Binding constant values and n values for the binding of Phosmet with BSA at three different temperatures confirmed non-covalent binding interactions with a single set of equivalent binding sites. Thermodynamic parameters ∆G (-137.40 ± 3.58 kJ mol-1); ΔH (-16.33 ± 5.28 kJ mol-1) and ΔS(-469 ± 12.45 kJ mol-1) confirmed that the binding was spontaneous and non-covalent interactions like electrostatic, hydrogen bonding and van der Waals forces played an important role in the binding. The CD data indicated the conformational change in BSA upon binding with Phosmet which resulted in a change in the melting temperature. Molecular docking presented the binding model for BSA-phosmet complex and displayed that non-covalent interactions played a significant role in the binding mechanism.
Microbial degradation of Phosmet on blueberry fruit and in aqueous systems by indigenous bacterial flora on lowbush blueberries (Vaccinium angustifolium)
J Food Sci 2007 Oct;72(8):M293-9.PMID:17995608DOI:10.1111/j.1750-3841.2007.00466.x.
Phosmet-adapted bacteria isolated from lowbush blueberries (Vaccinium angustifolium) were evaluated for their ability to degrade Phosmet on blueberry fruit and in minimal salt solutions. Microbial metabolism of Phosmet by isolates of Enterobacter agglomerans and Pseudomonas fluorescens resulted in significant reductions (P < 0.05; 33.8%) in Phosmet residues on blueberry fruit. Degradation was accompanied by microbial proliferation of phosmet-adapted bacteria. Preferential utilization of Phosmet as a carbon source was investigated in minimal salt solutions inoculated with either E. agglomerans or P. fluorescens and supplemented with Phosmet or Phosmet and glucose. Microbial degradation concurrent with the proliferation of P. fluorescens was similar in both liquid systems, indicative of preferential utilization of Phosmet as an energy substrate. E. agglomerans exhibited the ability to degrade Phosmet as a carbon source, yet in the presence of added glucose, Phosmet degradation occurred within the 1st 24 h only followed by total population mortality resulting in no appreciable degradation. Characteristic utilization of glucose by this isolate suggests a possible switch in carbon substrate utilization away from Phosmet, which resulted in toxicity from the remaining Phosmet. Overall, microbial metabolism of Phosmet as an energy source resulted in significant degradation of residues on blueberries and in minimal salt solutions. Thus, the role of adapted strains of E. agglomerans and P. fluorescens in degrading Phosmet on blueberries represents an extensive plant-microorganism relationship, which is essential to determination of Phosmet persistence under pre- and postharvest conditions.