Phosphatase Inhibitor Cocktail (2 Tubes, 100X)

Protein phosphorylation is an important covalent posttranslational modification that changes the conformation of proteins and regulates the function, location and specific binding of target proteins. Many cellular processes are regulated by reversible phosphorylation of proteins, and 30% of proteins may be phosphorylated in their presence.

Due to the balance of endogenous protein production and degradation, its cell level is stable under stable environmental conditions. The crude cell extract contains many endogenous enzymes, such as phosphatase and protease, which degrade and alter the proteins in the extract. The best way to increase complete protein production is to add inhibitors of these enzymes.

More inhibitors mean better phosphorylation protection. We offer two tubes of inhibitors with different solution forms to help you better inhibit phosphatase.

Tube A (phosatase Inhibitor Cocktail 1,  inhibits l-isozyme of alkaline Phosphatase and serine/threonine protein phosphatases, such as protein Phosphatase 1 and 2A (PP1 and PP2A). Tube A contains Cantharidin, Bromotetramisole and Microcystin LR, which are provided in the solution of DMSO.

Tube B (phosatase Inhibitor Cocktail 2,  inhibits tyrosine protein Phosphatase, acid Phosphatase and alkaline Phosphatase. Tube B contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole and Sodium Fluoride, with ddH2O box solution is provided.

蛋白质磷酸化是一种重要的共价后转录修饰，它改变了蛋白质的构象并调节目标蛋白质的功能、位置和特定结合。许多细胞过程都受到可逆性蛋白质磷酸化的调控，30% 的蛋白质可能在其存在下被磷酸化。

由于内源性蛋白质的产生和降解达到平衡，在稳定的环境条件下，其细胞水平是稳定的。粗细胞提取物中含有许多内源性酶，如磷酸酶和蛋白酶，它们会分解并改变提取物中的蛋白质。增加完整蛋白质产量的最佳方法是添加这些酶的抑制剂。

更多的抑制剂意味着更好的磷酸化保护。我们提供两种不同溶液形式的抑制剂管，以帮助您更好地抑制磷酸酶。

管子A（磷酸酶抑制剂混合物1）可以抑制碱性磷酸酶的l异构体和丝氨酸/苏氨酸蛋白磷酸酶，如蛋白质磷酸化1和2A（PP1和PP2A）。管子A含有坐果甲素、溴四唑和微囊藻毒素LR，这些成分都是在DMSO溶液中提供的。

管子B（磷酸酶抑制剂混合物2）可以抑制酪氨酸蛋白磷酸酶、酸性磷酸酶和碱性磷酸 酶。 管子B包含正硼钠，钼(VI) 钠，塔塔率钠，咪唑和氟化钠，并提供ddH2O盒装溶液。