Phosphatase Inhibitor Cocktail I (100× in DMSO)
目录号 : GK10011磷酸酶抑制剂混合物I可以有效地抑制碱性磷酸酶和丝氨酸/苏氨酸蛋白质磷酸酶，如PP1和PP2A。
Sample solution is provided at 25 µL, 10mM.
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Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).
Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Components
| Ingredient | M.W. | 100x Conc. | Target |
| (-)-p-Bromotetramisole oxalate | 995.2 | 2.5 mM | Alkaline phosphatases |
| Cantharidin | 373.2 | 500 μM | Ser/Thr phosphatases |
| Microcystin LR, Microcystis aeruginosa | 196.2 | 500 nM | PP1 and PP2A |
磷酸酶抑制剂混合物I可以有效地抑制碱性磷酸酶和丝氨酸/苏氨酸蛋白质磷酸酶，如PP1和PP2A。
蛋白质和脂质的磷酸化是细胞信号或代谢状态的标志。这种混合物由三种抑制剂组成，可以抑制碱性磷酸酶以及丝氨酸/苏氨酸蛋白磷酸酶如PP1和PP2A。每个小瓶中含有1毫升溶解在DMSO中的磷酸酶抑制剂混合物，包括以下成分：溴四唑草酰ate（2.5毫摩尔）、天仙子素（500微米）和微囊藻-LR，微囊藻属（500纳摩尔）。
磷酸酶抑制剂混合物I已经被优化并测试用于哺乳动物细胞和组织提取物。这种混合物以DMSO为基础，作为一种即用型溶液供应。