Phosphatase Inhibitor Cocktail II (100× in ddH₂O)
目录号 : GK10012磷酸酶抑制剂混合物II可以有效地抑制酸性和碱性磷酸酶以及蛋白质酪氨酸磷酸化酶(PTPs)。
Sample solution is provided at 25 µL, 10mM.
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Phosphatase Inhibitor Cocktail II effectively inhibits acid and alkaline phosphatases as well as protein tyrosine phosphatases(PTPs).
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents. Provided as 1 ml vial containing the following components: Imidazole (200 mM), Sodium Fluoride (100 mM), Sodium Molybdate (115 mM), Sodium Orthovanadate (100 mM), and Sodium Tartrate Dihydrate (400 mM).
Phosphatase Inhibitor Cocktail II has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in ddH2O.
Components
磷酸酶抑制剂混合物II可以有效地抑制酸性和碱性磷酸酶以及蛋白质酪氨酸磷酸化酶(PTPs)。
蛋白质和脂质的磷酸化是细胞信号或代谢状态的标志。这种混合物包含五种磷酸酶抑制剂,可用于抑制酸性和碱性磷酸酶以及蛋白质酪氨酸磷酸酶(PTPs)。适用于细胞裂解液和组织提取物,包括含有洗涤剂的样品。提供1毫升小瓶,其中包含以下成分:咪唑(200毫摩尔)、氟化钠(100毫摩尔)、钼酸钠(115毫摩尔)、正硼酸钠(100毫摩尔)和二水草酸钠盐(400毫摩尔)。
磷酸酶抑制剂混合物II已经针对哺乳动物细胞和组织提取物进行了优化和测试。该混合物以ddH2O为基础,作为一种即用型溶液供应。
组件
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
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