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Phosphine-biotin

(Synonyms: Click Tag Phosphine-biotin) 目录号 : GC44634

A probe for azido-labeled proteins

Phosphine-biotin Chemical Structure

Cas No.:608514-42-7

规格 价格 库存 购买数量
500μg
¥1,918.00
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1mg
¥3,649.00
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5mg
¥15,350.00
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Sample solution is provided at 25 µL, 10mM.

Description

Labeling reactive centers of various types in cells with specific site-directed probes is a common method to explore both function and biochemical modification of proteins. The popular click chemistry method of protein labeling employs use of a reaction between an azido group and an alkyne on complimentary pairs of a specific reactive probe and a labeling agent (i.e. a tag) such as biotin or a fluorophore. The Staudinger ligation is an alternative to the click chemistry reaction in which a phosphine-labeled molecule reacts with an azido group on the opposing molecule of interest. Phosphine biotin is a labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction. Modified proteins can be detected using common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE. For example, phosphine-biotin has been used successfully in conjunction with DAz-1 or DAz-2 to label and detect sulfenic acid sites in proteins.

Reference:
[1]. Seo, Y.H., and Carroll, K.S. Facile synthesis and biological evaluation of a cell-permeable probe to detect redox-regulated proteins. Bioorganic & Medicinal Chemistry Letters 19, 356-359 (2009).
[2]. Reddie, K.G., Seo, Y.H., Muse, W.B., III, et al. A chemical approach for detecting sulfenic acid-modified proteins in living cells. Molecular BioSystems 4, 521-531 (2008).

化学性质

Cas No. 608514-42-7 SDF
别名 Click Tag Phosphine-biotin
化学名 2-(diphenylphosphino)-4-[21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1,17-dioxo-6,9,12-trioxa-2,16-diazaheneicos-1-yl]-benzoic acid, methyl ester
Canonical SMILES O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCCOCCOCCOCCCNC(C3=CC=C(C(OC)=O)C(P(C4=CC=CC=C4)C5=CC=CC=C5)=C3)=O)=O)SC2)([H])N1
分子式 C41H53N4O8PS 分子量 792.9
溶解度 DMSO: 2 mg/ml 储存条件 Store at -20°C
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1 mg 5 mg 10 mg
1 mM 1.2612 mL 6.306 mL 12.6119 mL
5 mM 0.2522 mL 1.2612 mL 2.5224 mL
10 mM 0.1261 mL 0.6306 mL 1.2612 mL
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