Phosphine-biotin
(Synonyms: Click Tag Phosphine-biotin) 目录号 : GC44634A probe for azido-labeled proteins
Cas No.:608514-42-7
Sample solution is provided at 25 µL, 10mM.
Labeling reactive centers of various types in cells with specific site-directed probes is a common method to explore both function and biochemical modification of proteins. The popular click chemistry method of protein labeling employs use of a reaction between an azido group and an alkyne on complimentary pairs of a specific reactive probe and a labeling agent (i.e. a tag) such as biotin or a fluorophore. The Staudinger ligation is an alternative to the click chemistry reaction in which a phosphine-labeled molecule reacts with an azido group on the opposing molecule of interest. Phosphine biotin is a labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction. Modified proteins can be detected using common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE. For example, phosphine-biotin has been used successfully in conjunction with DAz-1 or DAz-2 to label and detect sulfenic acid sites in proteins.
Reference:
[1]. Seo, Y.H., and Carroll, K.S. Facile synthesis and biological evaluation of a cell-permeable probe to detect redox-regulated proteins. Bioorganic & Medicinal Chemistry Letters 19, 356-359 (2009).
[2]. Reddie, K.G., Seo, Y.H., Muse, W.B., III, et al. A chemical approach for detecting sulfenic acid-modified proteins in living cells. Molecular BioSystems 4, 521-531 (2008).
Cas No. | 608514-42-7 | SDF | |
别名 | Click Tag Phosphine-biotin | ||
化学名 | 2-(diphenylphosphino)-4-[21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1,17-dioxo-6,9,12-trioxa-2,16-diazaheneicos-1-yl]-benzoic acid, methyl ester | ||
Canonical SMILES | O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCCOCCOCCOCCCNC(C3=CC=C(C(OC)=O)C(P(C4=CC=CC=C4)C5=CC=CC=C5)=C3)=O)=O)SC2)([H])N1 | ||
分子式 | C41H53N4O8PS | 分子量 | 792.9 |
溶解度 | DMSO: 2 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 1.2612 mL | 6.306 mL | 12.6119 mL |
5 mM | 0.2522 mL | 1.2612 mL | 2.5224 mL |
10 mM | 0.1261 mL | 0.6306 mL | 1.2612 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet