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PHTPP Sale

目录号 : GC11863

An estrogen ERβ receptor antagonist

PHTPP Chemical Structure

Cas No.:805239-56-9

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10mg
¥900.00
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50mg
¥3,510.00
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Sample solution is provided at 25 µL, 10mM.

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实验参考方法

Cell experiment:

Cells are plated at a density of 3 to 4×105 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco’s Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37°C, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability[1].

References:

[1]. Compton DR, et al. Pyrazolo[1,5-a]pyrimidines: estrogen receptor ligands possessing estrogen receptor beta antagonist activity. J Med Chem. 2004 Nov 18;47(24):5872-93.
[2]. Ogo Y, et al. IGF-1 gene expression is differentially regulated by estrogen receptors α and β in mouse endometrial stromal cells and ovarian granulosa cells. J Reprod Dev. 2014;60(3):216-23. Epub 2014 Mar 25.

产品描述

PHTPP is a selective ERβ antagonist.

PHTPP is a selective ERβ antagonist. PHTPP reduces FSH-mediated cAMP production by 80% (pIgf1 mRNA expression, and facilitates the DPN-induced increase in class 1 Igf1 mRNA expression[2].

References:
[1]. Compton DR, et al. Pyrazolo[1,5-a]pyrimidines: estrogen receptor ligands possessing estrogen receptor beta antagonist activity. J Med Chem. 2004 Nov 18;47(24):5872-93.
[2]. Ogo Y, et al. IGF-1 gene expression is differentially regulated by estrogen receptors α and β in mouse endometrial stromal cells and ovarian granulosa cells. J Reprod Dev. 2014;60(3):216-23. Epub 2014 Mar 25.

Chemical Properties

Cas No. 805239-56-9 SDF
化学名 4-(2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl)phenol
Canonical SMILES FC(F)(C1=CC(C(F)(F)F)=NC2=C(C(C=C3)=CC=C3O)C(C4=CC=CC=C4)=NN21)F
分子式 C20H11F6N3O 分子量 423.31
溶解度 ≤30mg/ml in ethanol;50mg/ml in DMSO 储存条件 Store at RT
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 2.3623 mL 11.8117 mL 23.6233 mL
5 mM 0.4725 mL 2.3623 mL 4.7247 mL
10 mM 0.2362 mL 1.1812 mL 2.3623 mL
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