Phytosphingosine-d7
(Synonyms: D-ribo Phytosphingosine-d7,4-D-hydroxy Sphinganined7) 目录号 : GC91729Phytosphingosine-d7 is intended for use as an internal standard for the quantification of phytosphingosine by GC- or LC-MS.
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Phytosphingosine-d7 is intended for use as an internal standard for the quantification of phytosphingosine by GC- or LC-MS. Phytosphingosine is a sphingolipid with a hydroxyl group at the C4 position that is found mainly in fungi and plants but also in animals, including humans.[1],[2] It is metabolized to odd-numbered fatty acids with 2-hydroxy palmitic acid as an intermediate.[3] Phytosphingosine dose-dependently induces cell death of CHO cells and inhibits carbachol-induced activation of phospholipase D (PLD) in CHO cells transfected with C. elegans muscarinic acetylcholine receptors (mAChRs).[4] It is essential in the heat stress response in S. cerevisiae.[5]
References:
[1].Dickson, R.C.Sphingolipid functions in Saccharomyces cerevisiae: Comparison to mammalsAnnu. Rev. Biochem.6727-48(1998).
[2].Schürer, N.Y., Plewig, G., and Elias, P.M.Stratum corneum lipid functionDermatologica183(2)77-94(1991).
[3].Kondo, N., Ohno, Y., Yamagata, M., et al.Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acidsNat. Commun.55338(2014).
[4].Lee, J.S., Min, D.S., Park, C., et al.Phytosphingosine and C2-phytoceramide induce cell death and inhibit carbachol-stimulated phospholipase D activation in Chinese hamster ovary cells expressing the Caenorhabditis elegans muscarinic acetylcholine receptorFEBS. Lett.499(1-2)82-86(2001).
[5].Hannun, Y.A., Luberto, C., and Argraves, K.M.Enzymes of sphingolipid metabolism: From modular to integrative signalingBiochemistry40(16)4893-4903(2001).
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0807 mL | 15.4036 mL | 30.8071 mL |
| 5 mM | 0.6161 mL | 3.0807 mL | 6.1614 mL |
| 10 mM | 0.3081 mL | 1.5404 mL | 3.0807 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。