PI3Kb Human
目录号 : GP22583Phosphoinositide 3-kinase beta p110β/p85α Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 90.0% as determined by SDS Page. | Source | Sf9 insect cells. |
| Phycical Appearance | Sterile filtered liquid formualtion. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Phosphoinositide 3-kinase beta p110b/p85a; PI3Kb; PI3Kb. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Biological Activity | The specific activity was found to be 3,000 units/mg (1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute). | ||
| Formulation | 0.9 mg/ml solution in PBS and 2mM MgCl2. | ||
The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation ofPI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr,HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis.PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line.It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors.
The specific activity was found to be 3,000 units/mg (1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute).
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.