Polybrene
(Synonyms: 聚凝胺; Polybrene; 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide) 目录号 : GC19206
Polybrene是一种季铵盐,最早作为肝素中和剂被引入临床,此外,低剂量的Polybrene在体内和体外均被广泛用于提高病毒介导的基因转移效率[2-5]。
Cas No.:28728-55-4
Sample solution is provided at 25 µL, 10mM.
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A mixture of 920μL DMEM +40μL Polybrene (28728-55-4，Glpbio, USA,)+40μL 1 x 108TU/ml virus suspension was added to SH-SY5Y cells.
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First, 1.9 × 105 cells were seeded in six-well plates.After 12 h, a specific concentration of polybrene (#GC19206, GLPBIO), blank virus control at a certain amount of MOI, and a virus overexpressing NSUN5 were added.
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Approximately 72 h after transfection,lentivirus-rich supernatants were harvested, pooled, and then used to transduce HEK293T or SH-SY5Y cells in a 1:1 mixture with culture medium and the aid of 8 μg/mL polybrene (Catalog#28728-55-4; GLPBIO).
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Induced pluripotent stem cell (iPSC)-differentiated neuron system |
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Reaction Conditions |
0-32 µg/mL;24h |
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Applications |
Polybrene displays concentration-dependent effects. Starting at concentrations as low as 4 µg/mL, distinct neuritic beads emerge, whereas a concentration of 8 µg/mL induces neuritic fragmentation. |
| Animal experiment [2]: | |
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Animal models |
Adult (2-3 months) BALB/c mice |
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Preparation Method |
4 µg/µL polybrene was incubated at 37℃ for 15 min, and intracerebroventricularly (ICV) injection of 5 µL mixture was performed for 20 min. Rotating Rod test will take place in three days. |
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Dosage form |
4 µg/µL；ICV |
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Applications |
Polybrene injection resulted in mice exhibiting motor dysfunction on the third day. |
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References: [1]. Bao F, Shi H,et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003. |
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Polybrene, a quaternary ammonium salt, was firstly introduced into clinical practice as a heparin neutralizer[1]. Besides, polybrene at lower doses was popularly used to promote the efficiency of virus-mediated gene transfer both in vivo and in vitro[2-5]. Polybrene promote the binding of viruses on the cell surface by neutralizing the electrostatic repulsion between the opposing bilayers[6-7].
Polybrene(0-32 µg/mL;24h) displays concentration-dependent effects. Starting at concentrations as low as 4 µg/mL, distinct neuritic beads emerge, whereas a concentration of 8 µg/mL induces neuritic fragmentation[8]. Polyethylene(8µg/mL) significantly reduced the proliferation rate of Mesenchymal stem cells (MSCs) [9]. Polybrene markedly enhances the efficiency of lipofection and also compensates the serum-mediated inhibition of lipofection in a variety of cell lines[10].
Polybrene(4 µg/µL；ICV) injection resulted in mice exhibiting motor dysfunction on the third day.Polybrene induces neural death and gliosis in mice[8].
References:
[1]. Weiss.W. A, Gilman, J. S, et,al. Heparin neutralization with polybrene administered intravenously. J. Am. Med. Assoc. 166, 603-607 (1958).
[2]. Davis, H. E., Morgan, J. R. et,al. Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys. Chem. 97, 159-172 (2002).
[3]. Bellodi-Privato, M. et al. AAV gene transfer to the retina does not protect retrovirally transduced hepatocytes from the immune response. J. Mol. Med (Berl.). 82, 403-410 (2004).
[4]. Lauwers, E. et al. Neuropathology and neurodegeneration in rodent brain induced by lentiviral vector-mediated overexpression of alpha-synuclein. Brain Pathol. 13, 364-372 (2003).
[5]. Ferry, N., Duplessis, O., et,al. Retroviral-mediated gene transfer into hepatocytes in vivo. Proc. Natl Acad. Sci. USA 88, 8377-8381 (1991).
[6]. Sinn, P. L., Sauter, S. L. et,al. Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors--design, biosafety, and production. Gene Ther. 12, 1089-1098 (2005).
[7]. Kafri, T. Lentivirus vectors: difficulties and hopes before clinical trials. Curr. Opin. Mol. Ther. 3, 316-326 (2001).
[8]. Bao F, Shi H, et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003.
[9].Lin P, Correa D, et,al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891. doi: 10.1371/journal.pone.0023891. Epub 2011 Aug 26. PMID: 21887340; PMCID: PMC3162604.
[10].Abe A, Miyanohara A, Friedmann T. Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 May;5(5):708-11. doi: 10.1038/sj.gt.3300652. PMID: 9797877.
Polybrene是一种季铵盐,最早作为肝素中和剂被引入临床[1],此外,低剂量的Polybrene在体内和体外均被广泛用于提高病毒介导的基因转移效率[2-5]。Polybrene通过中和对立双分子层之间的静电斥力来促进病毒在细胞表面的结合[6-7]。
Polybrene(0 ~ 32 µg/mL;24h)呈浓度依赖性。从低至4 µg/mL的浓度开始,出现明显的神经炎珠,而8 µg/mL的浓度则引起神经炎碎片[8]。Polybrene (8µg/mL)显著降低间充质干细胞(Mesenchymal stem cells, MSCs)的增殖率[9]。在多种细胞系中,Polybrene可显著提高脂质感染的效率,并补偿血清介导的脂质感染抑制[10]。
Polybrene (4 µg/µL；ICV)注射后第3天小鼠出现运动功能障碍。并且Polybrene可以诱导小鼠神经死亡和神经胶质瘤[8]。
| Cas No. | 28728-55-4 | SDF | |
| 别名 | 聚凝胺; Polybrene; 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide | ||
| 分子式 | 分子量 | ||
| 溶解度 | 10mg/ml in Water | 储存条件 | Store at -20℃, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。