Polyethylenimine Linear(PEI) 40000（rapid lysis）
目录号 : GC26627
Cas No.:49533-93-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO.
PEI 40000 has many advantages over PEI 25000 Transfection Reagent. include:
1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral.
2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient.
This product is an instant type, which dissolves quickly and is easy to prepare.
Feature:
Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection
Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression
Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN
Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection
Cost Effective: Competitive transfection effect with more affordable prices
Application:
Cell Transfection
| Cas No. | 49533-93-7 | SDF | |
| 分子式 | (CH2CH2NH)n | 分子量 | 40,000 |
| 溶解度 | Soluble in water, insoluble in organic solvents: benzene, ether and acetone | 储存条件 | 2-8ºC |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 25 mL | 125 mL | 250 mL |
| 5 mM | 5 mL | 25 mL | 50 mL |
| 10 mM | 2.5 mL | 12.5 mL | 25 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。