Polyoxyethylene stearate (POES)
(Synonyms: 聚氧乙烯硬脂酸酯; POES) 目录号 : GC33151
聚氧乙烯硬脂酸酯（POES）（POES）是一种非离子型乳化剂。
Cas No.:9004-99-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | Polyoxyethylene 40 stearate is added to the test vinblastine solution. The cytotoxicity of vinblastine to K562/ADR cells is then assessed. The final concentrations of polyoxyethylene 40 stearate are 0, 50, 100, and 150 μg/mL. After 8 hours of treatment, cells are incubated for 4 hours in the presence of MTT reagent and then lysed with DMSO. Absorbance is measured at 490 nM[3]. |
Animal experiment: | Mice: Polyoxyethylene 40 stearate and vinblastine are dissolved in 0.9% sodium chloride solution, yielding a solution of 200 μg/mL VBL and 150 μg/mL PS40. The drug is injected subcutaneously in a volume of 0.1 mL per 10 g of body weight at a dosage of 2 mg/kg vinblastine and 1.5 mg/kg polyoxyethylene 40 stearate around the tumor every other day for 8 days. The volume of tumors and the weight of mice are measured every day from the day the tumors are formed[3]. |
References: [1]. Cutler RR, et al. The effect of polyoxyethylene stearate (POES) on the growth of mycobacteria in radiometric 7H12 Middlebrook TB medium. Tubercle. 1987 Sep;68(3):209-20. | |
Polyoxyethylene stearate (POES) is a non-ionic emulsifying agent.
Polyoxyethylene stearate has been recommended as an additive to the radiolabelled 7H12 Middlebrook TB media and as such has been shown to enhance growth of mycobacteria in the radiometric BACTEC rapid culture system. Polyoxyethylene (50) stearate produces the greatest enhancement in growth and reduction in the time taken to detect growth for M. tuberculosis and polyoxyethylene (30) stearate and polyoxyethylene (JL) stearate for species of mycobacteria other than M. tuberculosis[1]. Polyoxyethylene stearate inhibits P-gp mediated efflux in a concentration dependent manner mainly by modulating substrate-stimulated P-gp ATPase activity[2]. Polyoxyethylene 40 stearate reduces vinblastine sulfate efllux. The cytotoxicity of vinblastine to K562/ADR cells is significantly enhanced when the cells are cotreated with 100 or 150 μg/mL polyoxyethylene 40 stearate[3].
Polyoxyethylene stearate is potentially useful as a pharmaceutical ingredient to improve the oral bioavailability of coadministered P-gp substrates and substrates for certain CYP isoforms[2]. The average tumor volume and average tumor weight are significantly less in the polyoxyethylene 40 stearate+vinblastine group. The inhibition rate for tumor growth is increased from 0.06 (vinblastine group) to 0.84 (vinblastine+polyoxyethylene 40 stearate group)[3].
[1]. Cutler RR, et al. The effect of polyoxyethylene stearate (POES) on the growth of mycobacteria in radiometric 7H12 Middlebrook TB medium. Tubercle. 1987 Sep;68(3):209-20. [2]. Zhu S, et al. Effects of polyoxyethylene (40) stearate on the activity of P-glycoprotein and cytochrome P450. Eur J Pharm Sci. 2009 Jul 12;37(5):573-80. [3]. Luo L, et al. Polyoxyethylene 40 stearate modulates multidrug resistance and enhances antitumor activity of vinblastine sulfate. AAPS J. 2007 Oct 5;9(3):E329-35.
| Cas No. | 9004-99-3 | SDF | |
| 别名 | 聚氧乙烯硬脂酸酯; POES | ||
| Canonical SMILES | O=C(OCCO)CCCCCCCCCCCCCCCCC.[n].[n].[=].[10] | ||
| 分子式 | C20H40O3 | 分子量 | 328.53 |
| 溶解度 | DMSO : ≥ 49 mg/mL (149.15 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0439 mL | 15.2193 mL | 30.4386 mL |
| 5 mM | 0.6088 mL | 3.0439 mL | 6.0877 mL |
| 10 mM | 0.3044 mL | 1.5219 mL | 3.0439 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
The effect of Polyoxyethylene stearate (POES) on the growth of mycobacteria in radiometric 7H12 Middlebrook TB medium
Tubercle 1987 Sep;68(3):209-20.PMID:3448797DOI:10.1016/0041-3879(87)90057-2.
Polyoxyethylene stearate (POES) is a non-ionic emulsifying agent. Such agents have been shown to enhance the growth of mycobacteria in vitro. POES (POE(JL)S) has been recommended as an additive to the radiolabelled 7H12 Middlebrook TB media and as such has been shown to enhance growth of mycobacteria in the radiometric BACTEC rapid culture system. We examined the growth enhancing effect of six previously untested POE's (POE(8)S to POE(100)S) whose spreading properties (Hydrophilic-Lipophilic Balances-HLB's) were known. The aim of this study was to determine which POES has the greatest ability to enhance the growth of different mycobacterial species and how the HLB of each POES related to its effect on the growth of each species. We found that POE(50)S produced the greatest enhancement in growth and reduction in the time taken to detect growth for M. tuberculosis and POE(30)S and POE(JL)S for species of mycobacteria other than M. tuberculosis (MOTT). Comparing the effects of POES's to their HLB's (in BACTEC 7H12 media) we suggest four factors which may affect the growth enhancing ability of each POES: 1 its dispersal effect on the bacteria (acting as an emulsifier); 2 it's solubilizer effect on the bacterial cell; 3 the effect the POES on the dispersal of the radiolabelled fatty acid used as a substrate in the media to produce radiolabelled CO2; 4 mycobacteria may metabolise POES.
Growth inhibition of Mycobacterium tuberculosis by polyoxyethylene stearate present in the BACTEC pyrazinamide susceptibility test
J Clin Microbiol 1996 Jan;34(1):84-6.PMID:8748279DOI:10.1128/jcm.34.1.84-86.1996.
We have previously found that approximately 3.5% of 428 clinical isolates of Mycobacterium tuberculosis yield uninterpretable results in the BACTEC pyrazinamide (PZA) susceptibility test system, because of inadequate growth. We tested the hypothesis that Polyoxyethylene stearate (POES), the ingredient of the reconstituting fluid for the test, was the cause of this growth inhibition. A total of 15 isolates known for their previously uninterpretable results and 100 randomly chosen clinical isolates were tested in parallel both with and without POES. Repeat testing of the isolates with previously uninterpretable results yielded results in the presence of POES in only seven (47%). In the absence of POES, all gave interpretable results but one such result showed false resistance. For the other 100 clinical isolates, interpretable results were obtained with and without POES, but growth was enhanced in the absence of POES, especially in the PZA-susceptible strains. This was evidenced by a decreased time to attain a growth index of 200 in the control vial (4.9 days without POES versus 5.8 days with POES; P < 0.001) and a higher mean growth index ratio on the day of interpretation of the test (7.4% without POES versus 2.2% with POES; P < 0.001). However, the enhanced growth without POES led to 20 susceptible strains being misinterpreted as either resistant or borderline. We suggest that isolates of M. tuberculosis which yield uninterpretable results in the BACTEC PZA test system should be retested both with and without POES. If interpretable results indicating PZA resistance are obtained only in the absence of POES, the result should be confirmed by a pyrazinamidase assay or by the conventional proportion method. Routine omission of POES from the BACTEC test for all clinical strains is discouraged because of the unacceptably high false-resistance rates.
Inhibitory effects of polyoxyethylene stearate, PANTA, and neutral pH on growth of Mycobacterium genavense in BACTEC primary cultures
J Clin Microbiol 1997 Nov;35(11):2791-4.PMID:9350735DOI:10.1128/jcm.35.11.2791-2794.1997.
We report on the influences of Polyoxyethylene stearate (POES), PANTA, and pH on primary cultures of Mycobacterium genavense in BACTEC vials. As a model for primary cultures from tissue, seven different strains first isolated from AIDS patients (five from Switzerland and two from the United States) were inoculated into nude mice in order to obtain large amounts of bacilli to test different conditions simultaneously. Our results demonstrate that the size of the inoculum (10[6] acid-fast bacilli/vial), an acid pH (pH 6.0), and the absence of additives (POES and PANTA) significantly (P < 0.001) increased the probability of a successful culture in 1 month, considering growth index (GI) of > or =100 or a GI of > or =999 as criterion of success. In logistic regression analysis, all factors maintained a significant (P < 0.001) independent effect, and no interactions were observed between them. The best conditions for the primary cultures of M. genavense were the use of Middlebrook 7H12 medium at pH 6.0 without any additives.
Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention
Biotechnol Bioeng 2005 Jun 5;90(5):614-20.PMID:15818564DOI:10.1002/bit.20444.
Aggregation of polyethylenimine (PEI)-DNA complexes severely undermines their utility for gene delivery into mammalian cells. Herein we undertook to elucidate the mechanism of this deleterious phenomenon and to develop rational strategies for its prevention. The effect of temperature, surfactants, complex concentration, ionic strength, viscosity, and pH on the time course of this aggregation was systematically examined. The aggregation process was completely inhibited by 2.5% polyoxyethylene (100) stearate (POES) and to a lesser degree by other nonionic surfactants. Importantly, POES preserved the transfection efficiency of the complexes without inducing toxicity. The aggregation was also reduced by lowering the temperature and pH, diluting the complexes, and increasing the solution viscosity. It is concluded that PEI-DNA complexes aggregate primarily due to hydrophobic interactions, while electrostatic attractions play little role.
Controlled complexation of plasmid DNA with cationic polymers: effect of surfactant on the complexation and stability of the complexes
Colloids Surf B Biointerfaces 2008 Oct 1;66(1):77-83.PMID:18583110DOI:10.1016/j.colsurfb.2008.05.012.
The aggregation of the cationic polymer-plasmid DNA complexes of two commonly used polymers, polyethyleneimine (PEI) and poly-l-lysine (PLL) were systematically compared. The complexation was studied in 5% glucose solution at 25 degrees C using dynamic light scattering and isothermal titration calorimetry. The aggregation of the complexes was controlled by addition of the surfactant Polyoxyethylene stearate (POES). The stability of the complexes was evaluated using dextran sulphate (DS) as relaxing agent. The relaxation of the complexes in the presence of DS was studied using agarose gel electrophoresis. This study elucidates the role of surfactant in controlling the size of the PEI/pDNA complex and reveals the differences of the two polymers as complexing agents.