POR Human, Active
目录号 : GP26174POR produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 686 amino acids (1-680a
Sample solution is provided at 25 µL, 10mM.
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POR produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 686 amino acids (1-680a.a.) andhaving a molecular mass of 77.9kDa.POR is expressed with a 6 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Sf9,Baculovirus cells. |
| Phycical Appearance | Sterile filtered colorless solution. | Shipping Condition | withIcePacks |
| Synonyms | P450 (Cytochrome) Oxidoreductase, EC 1.6.2.4, CYPOR, P450R,CPR, NADPH-Dependent Cytochrome P450 Reductase, NADPH--Cytochrome P450Reductase, NADPH--cytochrome P450 reductase. | ||
| Amino Acid Sequence | MINMGDSHVDTSSTVSEAVA EEVSLFSMTD MILFSLIVGL LTYWFLFRKK KEEVPEFTKI QTLTSSVRES SFVEKMKKTGRNIIVFYGSQ TGTAEEFANR LSKDAHRYGM RGMSADPEEY DLADLSSLPE IDNALVVFCM ATYGEGDPTDNAQDFYDWLQ ETDVDLSGVK FAVFGLGNKT YEHFNAMGKY VDKRLEQLGA QRIFELGLGD DDGNLEEDFITWREQFWLAV CEHFGVEATG EESSIRQYEL VVHTDIDAAK VYMGEMGRLK SYENQKPPFD AKNPFLAAVTTNRKLNQGTE RHLMHLELDI SDSKIRYESG DHVAVYPAND SALVNQLGKI LGADLDVVMS LNNLDEESNKKHPFPCPTSY RTALTYYLDI TNPPRTNVLY ELAQYASEPS EQELLRKMAS SSGEGKELYL SWVVEARRHILAILQDCPSL RPPIDHLCEL LPRLQARYYS IASSSKVHPN SVHICAVVVE YETKAGRINK GVATNWLRAKEPVGENGGRA LVPMFVRKSQ FRLPFKATTP VIMVGPGTGV APFIGFIQER AWLRQQGKEV GETLLYYGCRRSDEDYLYRE ELAQFHRDGA LTQLNVAFSR EQSHKVYVQH LLKQDREHLW KLIEGGAHIY VCGDARNMARDVQNTFYDIV AELGAMEHAQ AVDYIKKLMT KGRYSLDVWS HHHHHH. | ||
| Stability | Store at 4буC if entire vial will be used within 2-4 weeks. Store, frozen at -20буC for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Biological Activity | Specific activity is > 4,000 pmol/min/mg. Defined by the amount of enzyme that reduction of 1 pmole cytochrome-C by NADPH/min. at pH-8 25C. | ||
| Formulation | POR protein solution (0.25mg/ml) contains Phosphatebuffer saline (pH 7.4) and 10% glycerol. | ||
P450 Oxidoreductase, also known as POR is a flavoprotein which contributeselectrons to all microsomal P450 enzymes.POR is localized to the endoplasmic reticulum, where it is also capableof transfering electrons to heme oxygenase as well as cytochrome b5. POR isstructurally related to two separate flavoprotein families; first one is ferredoxinnucleotide reductase and the second flavodoxin.
Specific activity is > 4,000 pmol/min/mg. Defined by the amount of enzyme that reduction of 1 pmole cytochrome-C by NADPH/min. at pH-8 25C.
Store at 4буC if entire vial will be used within 2-4 weeks. Store, frozen at -20буC for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.