Povidone iodine (iodopovidone)
(Synonyms: 聚乙烯吡咯烷酮碘; iodopovidone) 目录号 : GC32325
A microcide
Cas No.:25655-41-8
Sample solution is provided at 25 µL, 10mM.
Povidone-iodine is a microcide with broad-spectrum activity against bacteria, fungi, viruses, and protozoans.1 It reduces total anaerobic and aerobic bacterial counts in vitro in dog feces when applied topically as a 10% solution.2 Povidone-iodine decreases bacterial counts and the occurrence of anastomotic leaks in a dog model of emergent resection and anastomosis of the sigmoid colon when applied in situ at a concentration of 10% v/v. Topical administration of povidone-iodine (10% v/v) also reduces bacterial counts on the skin of cynomolgus monkeys.3 Formulations containing povidone-iodine have been used to disinfect skin before and after surgery.
1.Eggers, M., Eickmann, M., Kowalski, K., et al.Povidone-iodine hand wash and hand rub products demonstrated excellent in vitro virucidal efficacy against Ebola virus and modified vaccinia virus Ankara, the new European test virus for enveloped virusesBMC Infect. Dis.15:375(2015) 2.Jones, F.E., DeCosse, J.J., and Condon, R.E.Evaluation of "instant" preparation of the colon with povidone-iodineAnn. Surg.184(1)74-79(1976) 3.Nakata, H., Tsubotani, Y., Nii, T., et al.Effects of olanexidine gluconate on preoperative skin preparation: An experimental study in cynomolgus monkeysJ. Med. Microbiol.66(5)678-685(2017)
Cell experiment: | Each bacterial isolate is washed twice with phosphate-buffered saline (PBS, pH 7.2), centrifuged for 10 min at 1932xg at 20°C, and suspended in 3 mL of nutrient broth, adjusted to a turbidity equivalent of 0.5 McFarland standard. The bacterial suspension is diluted 1:100 with MHB to a final inoculum of 106 colony-forming units (cfu)/mL. For each bacterial strain, two rows of a 96-well microtitre plate are filled with the final bacterial inoculum (50 μL per well) and 50 μL of each serial dilution of ILαD. The procedure is repeated for the Povidone iodine (PVP-I) serial dilutions[1]. |
Animal experiment: | The mice are divided into 6 groups as follows: 1) saline+vehicle (control group), 2) Dp+vehicle, 3) Dp+BZK, 4) Dp+Povidone iodine (PVP-I), 5) Dp+Et-OH, and 6) Dp+CHG. Animals in the experimental groups are exposed to the allergen through the subcutaneous injection of 5 µg of Dp dissolving in 10 µL of saline in the ventral side of the right ear 2 to 3 days a week (a total of 8 times) under anesthesia with 4% halothane. Animals in the control group are not sensitized, receiving a subcutaneous injection of 10 µL of saline in the ventral side of the right ear. Animals receive an application of antiseptic agent are exposed to the allergen and treated with 0.2% (w/v) benzalkonium chloride (Dp+BZK), 10% (w/v) povidone-iodine (Dp+PVP-I), 80% (v/v) ethanol (Dp+Et-OH) or 0.5% (v/v) chlorhexidine gluconate (Dp+CHG). These agents are applied a total of 15 times during the experimental period. The BZK, Povidone iodine (PVP-I), Et-OH, and CHG are dissolved in 25 µL of injection water and applied gently to the dorsal side of the right ear using a micropipette with a fine plastic tip. The animals in the Dp+vehicle and control groups receive 25 µL of injection water. All animals are sacrificed on the last day of the experiment (day 18)[2]. |
References: [1]. Zisi AP, et al. Iodine-lithium-alpha-dextrin (ILαD) against Staphylococcus aureus skin infections: a comparative study of in-vitro bactericidal activity and cytotoxicity between ILαD and povidone-iodine. J Hosp Infect. 2017 Jul 20. pii: S0195-6701(17)30395-X. |
Cas No. | 25655-41-8 | SDF | |
别名 | 聚乙烯吡咯烷酮碘; iodopovidone | ||
Canonical SMILES | O=C1N(C(C)CC)CCC1.I[F,Cl,Br,I]I.[n].[n:x].[=].[10:1] | ||
分子式 | 分子量 | ||
溶解度 | DMSO : 15 mg/mL (Need ultrasonic); H2O : 2.4 mg/mL (Need ultrasonic) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
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