Procion Blue HB (Reactive Blue 2)
(Synonyms: 蓝色染料; Reactive Blue 2) 目录号 : GC33665
Procion Blue HB (Reactive Blue 2) (Reactive Blue 2) 是一种嘌呤能拮抗剂。
Cas No.:12236-82-7
Sample solution is provided at 25 µL, 10mM.
Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.
Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+ oscillations in HeLa cells. Reactive Blue 2 enhances a Ca2+ response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+ oscillations[1]. The application of Reactive Blue 2 increases K+ secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion[2].The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Ki of 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3]. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2[4]. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner[5].
[1]. Okuda A, et al. Reactive blue 2 induces calcium oscillations in HeLa cells. Jpn J Physiol. 2001 Jun;51(3):389-93. [2]. Lee JH, et al. Reactive blue 2, an antagonist of rat P2Y4, increases K+ secretion in rat cochlea strial marginal cells. Hear Res. 2006 Sep;219(1-2):66-73. [3]. Coughlan SJ, et al. Reactive blue 2 is a potent inhibitor of a thylakoid protein kinase. Eur J Biochem. 1991 Apr 23;197(2):467-71. [4]. Burnstock G, et al. P2-purinoceptors of two subtypes in the rabbit mesenteric artery: reactive blue 2 selectively inhibits responses mediated via the P2y-but not the P2x-purinoceptor. Br J Pharmacol. 1987 Feb;90(2):383-91. [5]. Uneyama H, et al. Suramin and reactive blue 2 are antagonists for a newly identified purinoceptor on rat megakaryocyte. Br J Pharmacol. 1994 Jan;111(1):245-9.
Cas No. | 12236-82-7 | SDF | |
别名 | 蓝色染料; Reactive Blue 2 | ||
Canonical SMILES | O=S(C(C(N)=C1C2=O)=CC(NC3=CC=C(NC4=NC(Cl)=NC(NC5=CC=CC(S(=O)(O)=O)=C5)=N4)C(S(=O)(O)=O)=C3)=C1C(C6=C2C=CC=C6)=O)(O)=O.O=S(C(C(N)=C7C8=O)=CC(NC9=CC=C(NC%10=NC(Cl)=NC(NC%11=CC=C(S(=O)(O)=O)C=C%11)=N%10)C(S(=O)(O)=O)=C9)=C7C(C%12=C8C=CC=C%12)=O)(O)=O | ||
分子式 | C29H20ClN7O11S3 | 分子量 | 774.16 |
溶解度 | DMSO : ≥ 110 mg/mL (142.09 mM);Water : 10 mg/mL (12.92 mM);Ethanol : < 1 mg/mL (insoluble) | 储存条件 | 4°C, protect from light |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
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1 mg | 5 mg | 10 mg |
1 mM | 1.2917 mL | 6.4586 mL | 12.9172 mL |
5 mM | 0.2583 mL | 1.2917 mL | 2.5834 mL |
10 mM | 0.1292 mL | 0.6459 mL | 1.2917 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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